Literature DB >> 11156556

Evidence for expression and function of phosphodiesterase type 5 (PDE-V) in rat resistance arteries.

L J Sampson1, J M Hinton, C J Garland.   

Abstract

Evidence is provided for expression and a functional role for phosphodiesterase type V (PDE-V) in the rat isolated small mesenteric artery. The reverse transcription polymerase chain reaction (RT--PCR) demonstrated mRNA for PDE-V, while Western blotting and immunocytochemical studies showed corresponding protein expression. Smooth muscle relaxation to the nitric oxide donor, diethylamine NONOate (DEA NONOate; 1 nM - 10 microM; pEC(50)=6.7+/-0.3) was potentiated significantly by the specific inhibitor of PDE-V, 4-[[3,4-(methylenedioxy)benzyl]amino]-6-chloroquinazoline (MBCQ; 1 microM; pEC(50)=10.5+/-0.04). These data show that PDE-V is expressed in both the smooth muscle and endothelial cells of a resistance artery, and the enzyme can significantly influence nitric oxide-evoked vasorelaxation.

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Year:  2001        PMID： 11156556      PMCID： PMC1572563          DOI： 10.1038/sj.bjp.0703831

Source DB:  PubMed          Journal:  Br J Pharmacol        ISSN： 0007-1188            Impact factor:   8.739


  12 in total

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2.  Characterisation of cyclic nucleotide phosphodiesterases from rat mesenteric artery.

Authors:  N Komas; C Lugnier; R Andriantsitohaina; J C Stoclet
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3.  Phosphodiesterase inhibition improves agonist-induced relaxation of hypertensive pulmonary arteries.

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4.  Gap junction localization and connexin expression in cytochemically identified endothelial cells of arterial tissue.

Authors:  H I Yeh; E Dupont; S Coppen; S Rothery; N J Severs
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5.  ANF elicits phosphorylation of the cGMP phosphodiesterase in vascular smooth muscle cells.

Authors:  T A Wyatt; A J Naftilan; S H Francis; J D Corbin
Journal:  Am J Physiol       Date:  1998-02

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Authors:  J Kotera; N Yanaka; K Fujishige; Y Imai; H Akatsuka; T Ishizuka; K Kawashima; K Omori
Journal:  Eur J Biochem       Date:  1997-10-15

7.  Expression of three isoforms of cGMP-binding cGMP-specific phosphodiesterase (PDE5) in human penile cavernosum.

Authors:  C S Lin; A Lau; R Tu; T F Lue
Journal:  Biochem Biophys Res Commun       Date:  2000-02-16       Impact factor: 3.575

8.  Genomic origin and transcriptional regulation of two variants of cGMP-binding cGMP-specific phosphodiesterases.

Authors:  J Kotera; K Fujishige; Y Imai; E Kawai; H Michibata; H Akatsuka; N Yanaka; K Omori
Journal:  Eur J Biochem       Date:  1999-06

9.  Sildenafil, a novel effective oral therapy for male erectile dysfunction.

Authors:  M Boolell; S Gepi-Attee; J C Gingell; M J Allen
Journal:  Br J Urol       Date:  1996-08

10.  Contribution of both nitric oxide and a change in membrane potential to acetylcholine-induced relaxation in the rat small mesenteric artery.

Authors:  G J Waldron; C J Garland
Journal:  Br J Pharmacol       Date:  1994-07       Impact factor: 8.739
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Authors:  Sutthinee Wisutthathum; Krongkarn Chootip; Hélène Martin; Kornkanok Ingkaninan; Prapapan Temkitthawon; Perle Totoson; Céline Demougeot
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