Literature DB >> 11153945

High-performance thin-layer chromatographic determination of potato glycoalkaloids.

B Simonovska1, I Vovk.   

Abstract

A high-performance thin-layer chromatographic (HPTLC) method for the determination of the two main potato glycoalkaloids, alpha-chaconine and alpha-solanine, in different parts of the potato plant, is described. Samples were extracted with diluted aqueous acetic acid containing the ion-pairing reagent 1-pentanesulfonic acid, sodium salt. Extracts were purified and analytes fivefold concentrated by a solid-phase extraction (SPE) procedure using a Sep-Pak C18 cartridge. The methanol SPE eluate was applied to the HPTLC silica gel 60 F254 plates. The separation was performed in chloroform-methanol-2% aqueous NH4OH (70:30:5) mixture as the mobile phase. Six different detection reagents: two modifications of the Dragendorff's reagent, phosphomolybdic acid, paraformaldehyde-phosphoric acid, Ce(IV)sulfate-sulfuric acid and sulfuric acid-ethanol reagents were applied for quantification by densitometry. Fluorescence enabled detection of 10 ng of each alkaloid.

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Year:  2000        PMID： 11153945     DOI： 10.1016/s0021-9673(00)00900-6

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  3 in total

1.  Evaluation of Solasonine Content and Expression Patterns 
of SGT1 Gene in Different Tissues of Two Iranian Eggplant (Solanum melongena L.) Genotypes.

Authors:  Mahmoud Bagheri; Ali Akbar Shahnejat Bushehri; Mohammad Reza Hassandokht; Mohammad Reza Naghavi
Journal:  Food Technol Biotechnol       Date:  2017-06       Impact factor: 3.918

2.  Liquid Chromatography Mass Spectrometry Quantification of α-solanine, α-chaconine, and Solanidine in Potato Protein Isolates.

Authors:  Søren D Nielsen; Jesper M Schmidt; Gitte H Kristiansen; Trine K Dalsgaard; Lotte B Larsen
Journal:  Foods       Date:  2020-04-02

3.  Antibacterial activity of the alkaloid-enriched extract from Prosopis juliflora pods and its influence on in vitro ruminal digestion.

Authors:  Edilene T Dos Santos; Mara Lúcia A Pereira; Camilla Flávia P G da Silva; Lourdes C Souza-Neta; Regina Geris; Dirceu Martins; Antônio Euzébio G Santana; Luiz Cláudio A Barbosa; Herymá Giovane O Silva; Giovana C Freitas; Mauro P Figueiredo; Fernando F de Oliveira; Ronan Batista
Journal:  Int J Mol Sci       Date:  2013-04-17       Impact factor: 5.923
  3 in total

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