Polymorphisms of the renin gene promoter in spontaneously hypertensive and Wistar-Kyoto rats.

1. In the present study, 1.39 kb of the renin gene 5' region in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was amplified by polymerase chain reaction from genomic DNA and sequenced. Consistent differences in the renin gene sequence of SHR and WKY rats were found at positions -725, -727, -979 and -1126/-1129 as numbered from the transcription start site (+1). No polymorphism was specific to hypertensive rats. 2. Gel-shift assays were performed using labelled SHR renin promotor DNA and nuclear proteins extracted from rat kidneys. The regions between -1122 and -1139 and between -701 and -797 showed protein binding.