| Literature DB >> 11151678 |
R ten Hoopen1, R Manteuffel, J Dolezel, L Malysheva, I Schubert.
Abstract
The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.Entities:
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Year: 2000 PMID: 11151678 DOI: 10.1007/s004120000109
Source DB: PubMed Journal: Chromosoma ISSN: 0009-5915 Impact factor: 4.316