BACKGROUND: The fate of exudative polymorphonuclear neutrophils (PMNs) at the local site after surgery is not well understood. We evaluated the fate and functions of exudative PMNs at the local site in patients who were undergoing major surgery. We also investigated the relation between PMN apoptosis and cytokine levels at the local site during the postoperative period. METHODS: Exudative PMNs were isolated from 11 patients during the postoperative period. Apoptosis, reactive oxygen intermediates (ROI) production, CD16, and tumor necrosis factor receptor expression of the PMNs were determined by flow cytometry. Cytokine levels in the drainage fluid were measured. RESULTS: Exudative PMN apoptosis was markedly inhibited on postoperative day 1 and then increased in a time-dependent manner. IL-6 and granulocyte macrophage colony-stimulating factor were significant factors to inhibit exudative PMN apoptosis; tumor necrosis factor-alpha and IL-10 were the factors to increase apoptosis. ROI production and CD16 expression of exudative PMNs were augmented when PMN apoptosis was inhibited in the early postoperative period. CONCLUSIONS: Exudative PMN apoptosis was inhibited after surgery; PMN function was augmented after surgery. Cytokines at the local site may modulate exudative PMN apoptosis. Exudative PMN apoptosis reflected the inflammatory response after surgery. Understanding the mechanisms of PMN apoptosis and its pathophysiologic significance at local inflammatory sites in vivo may help in the design of more rational treatments.
BACKGROUND: The fate of exudative polymorphonuclear neutrophils (PMNs) at the local site after surgery is not well understood. We evaluated the fate and functions of exudative PMNs at the local site in patients who were undergoing major surgery. We also investigated the relation between PMN apoptosis and cytokine levels at the local site during the postoperative period. METHODS: Exudative PMNs were isolated from 11 patients during the postoperative period. Apoptosis, reactive oxygen intermediates (ROI) production, CD16, and tumor necrosis factor receptor expression of the PMNs were determined by flow cytometry. Cytokine levels in the drainage fluid were measured. RESULTS: Exudative PMN apoptosis was markedly inhibited on postoperative day 1 and then increased in a time-dependent manner. IL-6 and granulocyte macrophage colony-stimulating factor were significant factors to inhibit exudative PMN apoptosis; tumor necrosis factor-alpha and IL-10 were the factors to increase apoptosis. ROI production and CD16 expression of exudative PMNs were augmented when PMN apoptosis was inhibited in the early postoperative period. CONCLUSIONS: Exudative PMN apoptosis was inhibited after surgery; PMN function was augmented after surgery. Cytokines at the local site may modulate exudative PMN apoptosis. Exudative PMN apoptosis reflected the inflammatory response after surgery. Understanding the mechanisms of PMN apoptosis and its pathophysiologic significance at local inflammatory sites in vivo may help in the design of more rational treatments.
Authors: Galina P Simeonova; Emil Slavov; Roustislav Usunov; Krasimira Halacheva; Dinco N Dinev Journal: Vet Res Commun Date: 2008-05-20 Impact factor: 2.459
Authors: Víctor Asensi; Eulalia Valle; Alvaro Meana; Joshua Fierer; Antonio Celada; Victoria Alvarez; José Paz; Eliecer Coto; José Antonio Carton; José Antonio Maradona; Angeles Dieguez; Julián Sarasúa; Marcos G Ocaña; José Manuel Arribas Journal: Infect Immun Date: 2004-07 Impact factor: 3.441
Authors: Robert Blomgran; Veronika Patcha Brodin; Deepti Verma; Ida Bergström; Peter Söderkvist; Christopher Sjöwall; Per Eriksson; Maria Lerm; Olle Stendahl; Eva Särndahl Journal: PLoS One Date: 2012-03-05 Impact factor: 3.240
Authors: Léa Fialkow; Luciano Fochesatto Filho; Mary C Bozzetti; Adriana R Milani; Edison M Rodrigues Filho; Roberta M Ladniuk; Paula Pierozan; Rafaela M de Moura; João C Prolla; Eric Vachon; Gregory P Downey Journal: Crit Care Date: 2006 Impact factor: 9.097