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Literature DB >> 11146097

Enzyme engineering: rational redesign versus directed evolution.

Abstract

Enzyme engineering is undergoing the most profound and exciting transformation in its history (1). It promises unprecedented expansion in the scope and applications of modified or improved enzymes with desired physical and catalytic properties. Two complementary strategies are currently available: rational redesign (2-5) and directed evolution (6,7). Although both approaches have met with great success, each has limitations. In this article, the perspectives for these enzyme-engineering strategies are discussed briefly.

Mesh：

Substances：
Enzymes

Year: 2001 PMID： 11146097 DOI： 10.1016/s0167-7799(00)01522-5

Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536

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Cited

17 in total

1. Synthetic pathway for production of five-carbon alcohols from isopentenyl diphosphate.

Authors: Howard H Chou; Jay D Keasling
Journal: Appl Environ Microbiol Date: 2012-08-31 Impact factor: 4.792

2. Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase.

Authors: Monal R Parikh; Ichiro Matsumura
Journal: J Mol Biol Date: 2005-09-23 Impact factor: 5.469

3. In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

Authors: Pengxiang Fan; Abigail M Miller; Anthony L Schilmiller; Xiaoxiao Liu; Itai Ofner; A Daniel Jones; Dani Zamir; Robert L Last
Journal: Proc Natl Acad Sci U S A Date: 2015-12-29 Impact factor: 11.205

4. Synthesis of short-chain diols and unsaturated alcohols from secondary alcohol substrates by the Rieske nonheme mononuclear iron oxygenase MdpJ.

Authors: Franziska Schäfer; Judith Schuster; Birgit Würz; Claus Härtig; Hauke Harms; Roland H Müller; Thore Rohwerder
Journal: Appl Environ Microbiol Date: 2012-06-29 Impact factor: 4.792

5. A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography.

Authors: Tarik F Massoud; Ramasamy Paulmurugan; Sanjiv S Gambhir
Journal: Nat Med Date: 2010-07-18 Impact factor: 53.440

6. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase.

Authors: Steven C Rothman; Mark Voorhies; Jack F Kirsch
Journal: Protein Sci Date: 2004-02-06 Impact factor: 6.725

Enzyme engineering: rational redesign versus directed evolution.

1. Synthetic pathway for production of five-carbon alcohols from isopentenyl diphosphate.

2. Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase.

3. In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network.

4. Synthesis of short-chain diols and unsaturated alcohols from secondary alcohol substrates by the Rieske nonheme mononuclear iron oxygenase MdpJ.

5. A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography.

6. Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase.

7. Folding Determinants of Transmembrane β-Barrels Using Engineered OMP Chimeras.

Review 8. Current advances in retroviral gene therapy.

9. HotSpot Wizard: a web server for identification of hot spots in protein engineering.

10. Modeling catalytic promiscuity in the alkaline phosphatase superfamily.