Circadian variation in the activity of the 5-HT(1B) autoreceptor in the region of the suprachiasmatic nucleus, measured by microdialysis in the conscious freely-moving rat.

Intracerebral microdialysis was used to examine the function of the terminal 5-hydroxytryptamine(1B) (5-HT(1B)) autoreceptor in the region of the suprachiasmatic nuclei (SCN) of freely moving conscious rats at six time points or zeitgeber times (ZTs) across the light:dark cycle. Infusion of the 5-HT(1A/1B) agonist 5-methoxy-3-(1,2,3,6-tetrahydro-4-pyridyl)-1H-indole (RU24969) (1 microM) via the microdialysis probe produced a decrease in 5-HT output when applied at ZTs 3, 6, 15 and 21 (69.8+/-11.9, 59+/-11.7, 43.9+/-17.2 and 45.7+/-17.0% respectively). At ZTs 9 and 18 RU24969 (1 microm) failed to affect the 5-HT output significantly (28.0+/-11 and 32.8+/-24.6% decrease respectively). The profile of inhibition of 5-HT output following infusion of RU24969 (1 microM) at ZT 6 was unaffected by concurrent infusion of the specific 5-HT(1A) antagonist N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl)cyclohe xanecarboxamide trihydrochloride (WAY100635) (1 microM) (52.48+/-17.5% decrease). The data demonstrate a circadian rhythm in the activity of the 5-HT(1B) autoreceptor in the region of the SCN.