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Literature DB >> 11136813

Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination.

Abstract

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

Entities: Species

Mesh：

Substances：
DNA, Protozoan

Year: 2001 PMID： 11136813 PMCID： PMC87744 DOI： 10.1128/JCM.39.1.401-403.2001

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

6 in total

Review 1. Variation in Cryptosporidium: towards a taxonomic revision of the genus.

Authors: U M Morgan; L Xiao; R Fayer; A A Lal; R C Thompson
Journal: Int J Parasitol Date: 1999-11 Impact factor: 3.981

2. Rapid and simple method for purification of nucleic acids.

Authors: R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal: J Clin Microbiol Date: 1990-03 Impact factor: 5.948

3. PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin.

Authors: F Spano; L Putignani; J McLauchlin; D P Casemore; A Crisanti
Journal: FEMS Microbiol Lett Date: 1997-05-15 Impact factor: 2.742

4. Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis.

Authors: J McLauchlin; S Pedraza-Díaz; C Amar-Hoetzeneder; G L Nichols
Journal: J Clin Microbiol Date: 1999-10 Impact factor: 5.948

5. Laboratory diagnosis of cryptosporidiosis.

Authors: D P Casemore; M Armstrong; R L Sands
Journal: J Clin Pathol Date: 1985-12 Impact factor: 3.411

6. Differentiating human from animal isolates of Cryptosporidium parvum.

Authors: I M Sulaiman; L Xiao; C Yang; L Escalante; A Moore; C B Beard; M J Arrowood; A A Lal
Journal: Emerg Infect Dis Date: 1998 Oct-Dec Impact factor: 6.883

6 in total

8 in total

1. Rapid and sensitive detection of single cryptosporidium oocysts from archived glass slides.

Authors: O Sunnotel; W J Snelling; L Xiao; K Moule; J E Moore; B Cherie Millar; J S G Dooley; C J Lowery
Journal: J Clin Microbiol Date: 2006-09 Impact factor: 5.948

2. Incidence of cryptosporidiosis species in paediatric patients in Malawi.

Authors: T D Morse; R A B Nichols; A M Grimason; B M Campbell; K C Tembo; H V Smith
Journal: Epidemiol Infect Date: 2007-01-15 Impact factor: 2.451

3. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces.

Authors: C F L Amar; P H Dear; S Pedraza-Díaz; N Looker; E Linnane; J McLauchlin
Journal: J Clin Microbiol Date: 2002-02 Impact factor: 5.948

4. HIV/AIDS-associated opportunistic protozoal diarrhea.

Authors: Mahmoud Agholi; Gholam Reza Hatam; Mohammad Hossein Motazedian
Journal: AIDS Res Hum Retroviruses Date: 2012-09-11 Impact factor: 2.205

5. Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa's solution-stained slides.

Authors: Kishor Pandey; Basu Dev Pandey; Arun Kumar Mallik; Osamu Kaneko; Haruki Uemura; Hiroji Kanbara; Tetsuo Yanagi; Kenji Hirayama
Journal: Parasitol Res Date: 2010-05-25 Impact factor: 2.289