Literature DB >> 11136129

PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins.

E Ben-Dov1, R Manasherob, A Zaritsky, Z Barak, Y Margalith.   

Abstract

An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

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Year:  2001        PMID： 11136129     DOI： 10.1007/s0028403336

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  7 in total

1.  An improved PCR-restriction fragment length polymorphism (RFLP) method for the identification of cry1-type genes.

Authors:  Changlong Shu; Dongming Liu; Zishan Zhou; Jilin Cai; Qi Peng; Jiguo Gao; Fuping Song; Jie Zhang
Journal:  Appl Environ Microbiol       Date:  2013-08-30       Impact factor: 4.792

2.  Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran.

Authors:  Gholamreza Salehi Jouzani; Ali Pourjan Abad; Ali Seifinejad; Rasoul Marzban; Khalil Kariman; Bahram Maleki
Journal:  J Ind Microbiol Biotechnol       Date:  2007-11-13       Impact factor: 3.346

3.  Cloning, characterization and diversity of insecticidal crystal protein genes of bacillus thuringiensis native isolates from soils of Andaman and Nicobar Islands.

Authors:  H M Mahadeva Swamy; R Asokan; S N Nagesha; D K Arora; Ajantha Birah; Riaz Mahmood
Journal:  Curr Microbiol       Date:  2011-08-21       Impact factor: 2.188

4.  New strategy for identification of novel Cry-type genes from Bacillus thuringiensis strains.

Authors:  Corina M Berón; Leonardo Curatti; Graciela L Salerno
Journal:  Appl Environ Microbiol       Date:  2005-02       Impact factor: 4.792

5.  Molecular characterization and genetic diversity of insecticidal crystal protein genes in native Bacillus thuringiensis isolates.

Authors:  H M Mahadeva Swamy; R Asokan; Riaz Mahmood; S N Nagesha
Journal:  Curr Microbiol       Date:  2012-12-04       Impact factor: 2.188

6.  Identification of cry1I-type genes from Bacillus thuringiensis strains and characterization of a novel cry1I-type gene.

Authors:  Fuping Song; Jie Zhang; Aixing Gu; Yue Wu; Lanlan Han; Kanglai He; Zhongyi Chen; Jiang Yao; Yuqin Hu; Guoxun Li; Dafang Huang
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

7.  The First Cry2Ac-Type Protein Toxic to Helicoverpa armigera: Cloning and Overexpression of Cry2ac7 Gene from SBS-BT1 Strain of Bacillus thuringiensis.

Authors:  Faiza Saleem; Abdul Rauf Shakoori
Journal:  Toxins (Basel)       Date:  2017-11-03       Impact factor: 4.546
  7 in total

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