Y Li1, Y Lou, Z Liang, H Ren. 1. Viral Hepatitis Institute of Chongqing University of Medical Sciences, Chongqing 400010, China.
Abstract
OBJECTIVE: To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg. METHODS: DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested. RESULTS: The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated. CONCLUSION: DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.
OBJECTIVE: To study the presenting effect of dendritic cell (DC) proliferated from peripheral blood monocytes in patients with chronic hepatitis B on HBsAg. METHODS: DC proliferated from patient's peripheral blood monocytes by adding GM-CSF and IL-4 were incubated with HBsAg for 1.5h at different concentrations, then mixed with autologous T cell and cocultured in 96-well flat-bottomed microtiter plates for 5 d at 37 degrees C and 5% CO(2). (3)H-TdR was added 13h before the culture completion, and finally the cells were gathered and the cpm was tested. RESULTS: The proliferation of T cell by DC treated by HBsAg is significantly higher than untreated. CONCLUSION: DC cultured from peripheral blood monocytes in patients with chronic hepatitis B plays a role in presenting HBsAg.