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Literature DB >> 1113376

Method for determination of nucleotide sequence homology between viral genomes by DNA reassociation kinetics.

Abstract

A model and appropriate equations were derived for the quantitative estimation of nucleotide sequence homology between two partially related viral genomes by measurement of the initial rate of reassociation of one labeled DNA in the presence of a second unlabeled DNA. The validity and usefulness of this procedure were demonstrated by the analysis of the reassociation kinetics of labeled adenovirus 7 DNA in the presence of unlabeled adenovirus 2 DNA. Based on DNA reassociation, the extent of homology between adenovirus 2 and 7 genomes was found to be 10 to 12%. The duplex formed between adenovirus 2 and 7 DNA had the appropriate thermal stability for a well-matched DNA-DNA hybrid.

Entities: Species

Mesh：

Substances：
DNA, Viral
Tritium

Year: 1975 PMID： 1113376 PMCID： PMC354481

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

11 in total

1. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA.

Authors: M GREEN; M PINA
Journal: Proc Natl Acad Sci U S A Date: 1964-06 Impact factor: 11.205

2. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.

Authors: M GREEN; M PINA
Journal: Virology Date: 1963-05 Impact factor: 3.616

3. Mapping of base sequence heterologies between genomes from different adenovirus serotypes.

Authors: C F Garon; K W Berry; J C Hierholzer; J A Rose
Journal: Virology Date: 1973-08 Impact factor: 3.616

4. Viral RNA subunits in cells transformed by RNA tumor viruses.

Authors: N Tsuchida; M S Robin; M Green
Journal: Science Date: 1972-06-30 Impact factor: 47.728

5. Detection of avian tumor virus-specific nucleotide sequences in avian cell DNAs (reassociation kinetics-RNA tumor viruses-gas antigen-Rous sarcoma virus, chick cells).

Authors: H E Varmus; R A Weiss; R R Friis; W Levinson; J M Bishop
Journal: Proc Natl Acad Sci U S A Date: 1972-01 Impact factor: 11.205

6. Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells.

Authors: L D Gelb; S A Aaronson; M A Martin
Journal: Science Date: 1971-06-25 Impact factor: 47.728

7. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes.

Authors: L D Gelb; D E Kohne; M A Martin
Journal: J Mol Biol Date: 1971-04-14 Impact factor: 5.469

8. RD-114 virus: analysis of viral gene sequences in feline and human cells by DNA-DNA reassociation kinetics and RNA-DNA hybridization.

Authors: K Fujinaga; A Rankin; H Yamazaki; K Sekikawa; J Bragdon; M Green
Journal: Virology Date: 1973-12 Impact factor: 3.616

9. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.

Authors: R J Britten; D E Kohne
Journal: Science Date: 1968-08-09 Impact factor: 47.728

10. The mechanism of viral carcinogenesis by DNA mammalian viruses: DNA-DNA homology relationships among the "weakly" oncogenic human adenoviruses.

Authors: S La; M Green
Journal: J Gen Virol Date: 1967-10 Impact factor: 3.891

4 in total