Inheritance of allozyme loci in Bombina: second linkage group established.

Segregation and linkage relationship of nine allozyme loci, which are fixed for alternative alleles in the European fire-bellied toads, Bombinal bombina and B. variegata, were studied using artificial F1 hybrids to obtain backcross and F2 progeny. Alleles coding for electromorphs at nine loci (Ldh-1, Mdh-1, Idh-1, Ck, Ak, Gpi, Aat-1, Np, and G6pd) showed Mendelian ratios. Two of the loci, Ak and G6pd, were found to be closely linked (2 cM apart); the other loci assorted independently.