B A Wambach1, H Cheung, G D Josephson. 1. Department of Otolaryngology--Head and Neck Surgery, University of Miami, Florida, USA.
Abstract
OBJECTIVE/HYPOTHESIS: Reconstructive procedures of the head and neck often require materials that offer long-term structural support. A problem that many surgeons have encountered is identifying a material that offers this support without rejection of the implanted material This has led many surgeons to prefer autologous cartilage However, autologous cartilage is of limited supply. Cartilage tissue engineering has become a new modality that allows investigators to harvest a small piece of cartilage and extract its chondrocytes for expansion in culture. These chondrocytes are applied to a matrix that will act as a scaffold and allow for cartilage growth. Finding a compatible matrix seems to be the limiting step in the progress of this research. We describe a new approach of tissue creation using bovine collagen matrices as templates onto which cells are seeded. STUDY DESIGN: Laboratory research. METHODS: Chondrocytes obtained from thyroid cartilage of dogs were seeded onto bovine collagen type I matrices and grown in vitro. Chondrocyte seeded matrices were evaluated histologically. RESULTS: Thyroid chondrocytes expressed themselves phenotypically by producing type II collagen in the presence of this type I collagen matrix. CONCLUSIONS: This study offers the preliminary findings on an exciting new type of matrix worth exploring in the ability to successfully engineer cartilage.
OBJECTIVE/HYPOTHESIS: Reconstructive procedures of the head and neck often require materials that offer long-term structural support. A problem that many surgeons have encountered is identifying a material that offers this support without rejection of the implanted material This has led many surgeons to prefer autologous cartilage However, autologous cartilage is of limited supply. Cartilage tissue engineering has become a new modality that allows investigators to harvest a small piece of cartilage and extract its chondrocytes for expansion in culture. These chondrocytes are applied to a matrix that will act as a scaffold and allow for cartilage growth. Finding a compatible matrix seems to be the limiting step in the progress of this research. We describe a new approach of tissue creation using bovine collagen matrices as templates onto which cells are seeded. STUDY DESIGN: Laboratory research. METHODS: Chondrocytes obtained from thyroid cartilage of dogs were seeded onto bovine collagen type I matrices and grown in vitro. Chondrocyte seeded matrices were evaluated histologically. RESULTS: Thyroid chondrocytes expressed themselves phenotypically by producing type II collagen in the presence of this type I collagen matrix. CONCLUSIONS: This study offers the preliminary findings on an exciting new type of matrix worth exploring in the ability to successfully engineer cartilage.
Authors: Serafim M Oliveira; Rushali A Ringshia; Racquel Z Legeros; Elizabeth Clark; Michael J Yost; Louis Terracio; Cristina C Teixeira Journal: J Biomed Mater Res A Date: 2010-08 Impact factor: 4.396