Literature DB >> 11128641

Synthesis of a statistically exhaustive fluorescent peptide substrate library for profiling protease specificity.

J E Sheppeck1, H Kar, L Gosink, J B Wheatley, E Gjerstad, S M Loftus, A R Zubiria, J W Janc.   

Abstract

A statistically exhaustive, 8800 compound tripeptidal amidomethylcoumarin library was synthesized as discreet compounds using solid-phase combinatorial chemistry. A subset of the compounds was purified by HPLC and tested in a high-throughput fluorometric assay against several known serine and cysteine proteases to demonstrate the utility of this library for profiling protease substrate specificity.

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Year:  2000        PMID： 11128641     DOI： 10.1016/s0960-894x(00)00545-x

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  3 in total

1.  Distinct specificities of Mycobacterium tuberculosis and mammalian proteasomes for N-acetyl tripeptide substrates.

Authors:  Gang Lin; Christopher Tsu; Lawrence Dick; Xi K Zhou; Carl Nathan
Journal:  J Biol Chem       Date:  2008-10-01       Impact factor: 5.157

2.  Cleavage Specificity of Mycobacterium tuberculosis ClpP1P2 Protease and Identification of Novel Peptide Substrates and Boronate Inhibitors with Anti-bacterial Activity.

Authors:  Tatos Akopian; Olga Kandror; Christopher Tsu; Jack H Lai; Wengen Wu; Yuxin Liu; Peng Zhao; Annie Park; Lisa Wolf; Lawrence R Dick; Eric J Rubin; William Bachovchin; Alfred L Goldberg
Journal:  J Biol Chem       Date:  2015-03-10       Impact factor: 5.157

3.  Target Validation and Identification of Novel Boronate Inhibitors of the Plasmodium falciparum Proteasome.

Authors:  Stanley C Xie; David L Gillett; Natalie J Spillman; Christopher Tsu; Madeline R Luth; Sabine Ottilie; Sandra Duffy; Alexandra E Gould; Paul Hales; Benjamin A Seager; Carlie L Charron; Frank Bruzzese; Xiaofeng Yang; Xiansi Zhao; Shih-Chung Huang; Craig A Hutton; Jeremy N Burrows; Elizabeth A Winzeler; Vicky M Avery; Lawrence R Dick; Leann Tilley
Journal:  J Med Chem       Date:  2018-11-07       Impact factor: 7.446
  3 in total

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