H Chart1, J S Cheesbrough, D J Waghorn. 1. Laboratory of Enteric Pathogens, Division of Gastrointestinal Infections, Central Public Health Laboratory, 61 Colindale Avenue, London, NW9 5HT, UK. hchart@nhs.co.uk
Abstract
BACKGROUND/AIMS: The serodiagnosis of infection with Salmonella typhi, using the Widal agglutination assay, relies on patients' antibodies to the O = 9,12 lipopolysaccharide (LPS) antigens, H = d flagellar antigens, and the Vi capsular antigens. A Vi agglutination titre of > 1/40 has traditionally been regarded as indicative of recent infection with S typhi. In this study, 91 sera were used to assess the reliability of the Widal agglutination assay based on antibodies to the Vi antigens. METHODS: The Widal agglutination assay was carried out using protocols established by the Central Public Health Laboratory, Colindale. Antibodies to the Vi capsular antigen were detected using a standard preparation of S typhi, ViI Bhatnagar variant strain (S typhi, ViI). Sera used in the study comprised 73 from patients who were culture positive for S typhi, 10 from patients who were culture positive for other species of Salmonella not expressing a Vi antigen (namely, S javiana, S enteritidis, S typhimurium, S stanley, S saint paul, S bareilly, or S mbandaka), and eight from healthy blood donors. RESULTS: Agglutination titres of > or = 1/40 were detected to S typhi ViI in 69 of 73 sera from patients with typhoid, although 27 of these also agglutinated an unrelated control antigen. The Widal assay also detected significant amounts of agglutinating antibodies to S. typhi ViI in all eight control sera and seven sera from patients infected with S bareilly, S enteritidis, S javiana, S mbandaka, S saint paul, and S stanley. CONCLUSIONS: Agglutinating antibodies to the Vi antigen can be detected by the Widal assay, but even with the appropriate control antigens the results were unreliable. The serodiagnosis of infections with S typhi should be based on the detection of antibodies to both the O = 9,12 LPS antigen and the H = d flagellar antigen by immunoblotting, and should not use the Vi antigen-based Widal assay. Conclusions should be made in the light of patients' clinical details and any knowledge of previous immunisation for typhoid.
BACKGROUND/AIMS: The serodiagnosis of infection with Salmonella typhi, using the Widal agglutination assay, relies on patients' antibodies to the O = 9,12 lipopolysaccharide (LPS) antigens, H = d flagellar antigens, and the Vi capsular antigens. A Vi agglutination titre of > 1/40 has traditionally been regarded as indicative of recent infection with S typhi. In this study, 91 sera were used to assess the reliability of the Widal agglutination assay based on antibodies to the Vi antigens. METHODS: The Widal agglutination assay was carried out using protocols established by the Central Public Health Laboratory, Colindale. Antibodies to the Vi capsular antigen were detected using a standard preparation of S typhi, ViI Bhatnagar variant strain (S typhi, ViI). Sera used in the study comprised 73 from patients who were culture positive for S typhi, 10 from patients who were culture positive for other species of Salmonella not expressing a Vi antigen (namely, S javiana, S enteritidis, S typhimurium, S stanley, S saint paul, S bareilly, or S mbandaka), and eight from healthy blood donors. RESULTS: Agglutination titres of > or = 1/40 were detected to S typhi ViI in 69 of 73 sera from patients with typhoid, although 27 of these also agglutinated an unrelated control antigen. The Widal assay also detected significant amounts of agglutinating antibodies to S. typhi ViI in all eight control sera and seven sera from patients infected with S bareilly, S enteritidis, S javiana, S mbandaka, S saint paul, and S stanley. CONCLUSIONS: Agglutinating antibodies to the Vi antigen can be detected by the Widal assay, but even with the appropriate control antigens the results were unreliable. The serodiagnosis of infections with S typhi should be based on the detection of antibodies to both the O = 9,12 LPS antigen and the H = d flagellar antigen by immunoblotting, and should not use the Vi antigen-based Widal assay. Conclusions should be made in the light of patients' clinical details and any knowledge of previous immunisation for typhoid.
Authors: R L Buck; J Escamilla; R P Sangalang; A B Cabanban; L T Santiago; C P Ranoa; J H Cross Journal: Trans R Soc Trop Med Hyg Date: 1987 Impact factor: 2.184
Authors: S L Hoffman; T P Flanigan; D Klaucke; B Leksana; R C Rockhill; N H Punjabi; S P Pulungsih; A Sutomo; M A Moechtar Journal: Am J Epidemiol Date: 1986-05 Impact factor: 4.897
Authors: Jessica Zuñiga; Luis Lillo; Junghee J Shin; Rajya L Machavarapu; Teresa Quiroga; Manuela Goycoolea; Betty Matsuhiro; L Aron-Hott; Henry P Godfrey; Felipe C Cabello Journal: J Clin Microbiol Date: 2005-09 Impact factor: 5.948
Authors: Benedikt Ley; George Mtove; Kamala Thriemer; Ben Amos; Lorenz von Seidlein; Ilse Hendriksen; Abraham Mwambuli; Aikande Shoo; Rajabu Malahiyo; Shaali M Ame; Deok R Kim; Leon R Ochiai; John D Clemens; Hugh Reyburn; Harald Wilfing; Stephen Magesa; Jacqueline L Deen Journal: BMC Infect Dis Date: 2010-06-22 Impact factor: 3.090
Authors: Corinne N Thompson; Mike Kama; Shrish Acharya; Una Bera; John Clemens; John A Crump; Aggie Dawainavesi; Gordon Dougan; W John Edmunds; Kimberley Fox; Kylie Jenkins; M Imran Khan; Josefa Koroivueta; Myron M Levine; Laura B Martin; Eric Nilles; Virginia E Pitzer; Shalini Singh; Ratu Vereniki Raiwalu; Stephen Baker; Kim Mulholland Journal: Trop Med Int Health Date: 2014-07-25 Impact factor: 2.622
Authors: Helen L Storey; Ying Huang; Chris Crudder; Allison Golden; Tala de los Santos; Kenneth Hawkins Journal: PLoS One Date: 2015-11-13 Impact factor: 3.240