Literature DB >> 11122533

Using an enzyme linked immunosorbent assay (ELISA) and a protein phosphatase inhibition assay (PPIA) for the detection of microcystins and nodularins.

W W Carmichael1, J An.   

Abstract

Cyanotoxins produced by cyanobacteria (blue-green algae) include potent neurotoxins and hepatotoxins. The hepatotoxins include cyclic peptide microcystins and nodularins plus the alkaloid cylindrospermopsins. Among the cyanotoxins the microcystins have proven to be the most widespread, and are most often implicated in animal and human poisonings. This paper presents a practical guide to two widely used methods for detecting and quantifying microcystins and nodularins in environmental samples-the enzyme linked immunosorbant assay (ELISA) and the protein phosphatase inhibition assay (PPIA).

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Year:  1999        PMID： 11122533     DOI： 10.1002/1522-7189(199911/12)7:6<377::aid-nt80>3.0.co;2-8

Source DB:  PubMed          Journal:  Nat Toxins        ISSN： 1056-9014


  22 in total

1.  Molecular characterization of potential microcystin-producing cyanobacteria in Lake Ontario embayments and nearshore waters.

Authors:  A M Hotto; M F Satchwell; G L Boyer
Journal:  Appl Environ Microbiol       Date:  2007-05-25       Impact factor: 4.792

2.  Mechanistic study and the influence of oxygen on the photosensitized transformations of microcystins (cyanotoxins).

Authors:  Weihua Song; Sabrina Bardowell; Kevin E O'Shea
Journal:  Environ Sci Technol       Date:  2007-08-01       Impact factor: 9.028

3.  Identifying the source of unknown microcystin genes and predicting microcystin variants by comparing genes within uncultured cyanobacterial cells.

Authors:  Christopher J Allender; Gary R LeCleir; Johanna M Rinta-Kanto; Randall L Small; Michael F Satchwell; Gregory L Boyer; Steven W Wilhelm
Journal:  Appl Environ Microbiol       Date:  2009-04-10       Impact factor: 4.792

4.  Widespread distribution and identification of eight novel microcystins in antarctic cyanobacterial mats.

Authors:  Susanna A Wood; Doug Mountfort; Andrew I Selwood; Patrick T Holland; Jonathan Puddick; S Craig Cary
Journal:  Appl Environ Microbiol       Date:  2008-10-10       Impact factor: 4.792

5.  Occurrence of cyanobacteria and microcystin toxins in raw and treated waters of the Nile River, Egypt: implication for water treatment and human health.

Authors:  Zakaria A Mohamed; Mohamed Ali Deyab; Mohamed I Abou-Dobara; Ahmad K El-Sayed; Wesam M El-Raghi
Journal:  Environ Sci Pollut Res Int       Date:  2015-04-10       Impact factor: 4.223

6.  Comparative protein expression in different strains of the bloom-forming cyanobacterium Microcystis aeruginosa.

Authors:  Ralitza Alexova; Paul A Haynes; Belinda C Ferrari; Brett A Neilan
Journal:  Mol Cell Proteomics       Date:  2011-05-24       Impact factor: 5.911

7.  Cyanotoxin bioaccumulation in freshwater fish, Washington State, USA.

Authors:  F Joan Hardy; Art Johnson; Kathy Hamel; Ellen Preece
Journal:  Environ Monit Assess       Date:  2015-10-05       Impact factor: 2.513

8.  Biochemical and morphological biomarkers of the liver damage in the Neotropical fish, Piaractus mesopotamicus, injected with crude extract of cyanobacterium Radiocystis fernandoi.

Authors:  Driele Tavares; Marcelo Gustavo Paulino; Ana Paula Terezan; João Batista Fernandes; Alessandra Giani; Marisa Narciso Fernandes
Journal:  Environ Sci Pollut Res Int       Date:  2018-03-21       Impact factor: 4.223

9.  Polysaccharides as a protective response against microcystin-induced oxidative stress in Chlorella vulgaris and Scenedesmus quadricauda and their possible significance in the aquatic ecosystem.

Authors:  Zakaria A Mohamed
Journal:  Ecotoxicology       Date:  2008-04-04       Impact factor: 2.823

10.  Biodegradation of microcystin-RR by Bacillus flexus isolated from a Saudi freshwater lake.

Authors:  Saad A Alamri
Journal:  Saudi J Biol Sci       Date:  2012-07-04       Impact factor: 4.219
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