Literature DB >> 11121763

Transposon-based approaches to identify essential bacterial genes.

N Judson1, J J Mekalanos.   

Abstract

Transposons are a powerful tool for identifying genes essential for bacterial viability. The availability of many bacterial genome sequences and the large number of genes of unknown function therein have inspired the generation of a variety of different approaches. These methods are described and their advantages and disadvantages are discussed.

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Year:  2000        PMID： 11121763     DOI： 10.1016/s0966-842x(00)01865-5

Source DB:  PubMed          Journal:  Trends Microbiol        ISSN： 0966-842X            Impact factor:   17.079


  30 in total

1.  Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.

Authors:  Jane A Thanassi; Sandra L Hartman-Neumann; Thomas J Dougherty; Brian A Dougherty; Michael J Pucci
Journal:  Nucleic Acids Res       Date:  2002-07-15       Impact factor: 16.971

2.  Chromosomal deletion formation system based on Tn5 double transposition: use for making minimal genomes and essential gene analysis.

Authors:  Igor Y Goryshin; Todd A Naumann; Jennifer Apodaca; William S Reznikoff
Journal:  Genome Res       Date:  2003-03-12       Impact factor: 9.043

Review 3.  Determination of the core of a minimal bacterial gene set.

Authors:  Rosario Gil; Francisco J Silva; Juli Peretó; Andrés Moya
Journal:  Microbiol Mol Biol Rev       Date:  2004-09       Impact factor: 11.056

4.  Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli.

Authors:  Mikhail Bubunenko; Teresa Baker; Donald L Court
Journal:  J Bacteriol       Date:  2007-02-02       Impact factor: 3.490

5.  Essential genes in Salmonella enteritidis as identified by TnAraOut mutagenesis.

Authors:  Jeong Nam Kim; Gun Woong Youm; Young Min Kwon
Journal:  Curr Microbiol       Date:  2008-08-14       Impact factor: 2.188

6.  Vibrio cholerae represses polysaccharide synthesis to promote motility in mucosa.

Authors:  Zhenyu Liu; Yuning Wang; Shengyan Liu; Ying Sheng; Karl-Gustav Rueggeberg; Hui Wang; Jie Li; Frank X Gu; Zengtao Zhong; Biao Kan; Jun Zhu
Journal:  Infect Immun       Date:  2015-01-05       Impact factor: 3.441

7.  Identification of essential genes in the human fungal pathogen Aspergillus fumigatus by transposon mutagenesis.

Authors:  Arnaud Firon; François Villalba; Roland Beffa; Christophe D'Enfert
Journal:  Eukaryot Cell       Date:  2003-04

8.  From genetic footprinting to antimicrobial drug targets: examples in cofactor biosynthetic pathways.

Authors:  Svetlana Y Gerdes; Michael D Scholle; Mark D'Souza; Axel Bernal; Mark V Baev; Michael Farrell; Oleg V Kurnasov; Matthew D Daugherty; Faika Mseeh; Boris M Polanuyer; John W Campbell; Shubha Anantha; Konstantin Y Shatalin; Shamim A K Chowdhury; Michael Y Fonstein; Andrei L Osterman
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

9.  Compromised outer membrane integrity in Vibrio cholerae Type II secretion mutants.

Authors:  Aleksandra E Sikora; Suzanne R Lybarger; Maria Sandkvist
Journal:  J Bacteriol       Date:  2007-09-21       Impact factor: 3.490

10.  Isolation of antibiotic hypersusceptibility mutants of Acinetobacter spp. by selection for DNA release.

Authors:  Hyunwoo Lee; Nora Vázquez-Laslop; Katya A Klyachko; Alex A Neyfakh
Journal:  Antimicrob Agents Chemother       Date:  2003-04       Impact factor: 5.191
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