OBJECTIVE: To investigate the effect of human oviductal cell coculture on the incidence of apoptosis in mouse embryos. DESIGN: Experimental laboratory study. SETTING: University gynecology unit. PATIENT(S): Fallopian tubes were obtained from patients undergoing hysterectomy. INTERVENTION(S): Mouse embryos were cocultured with human oviductal cells. MAIN OUTCOME MEASURE(S): Blastocyst development, allocation of inner cell mass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos. RESULT(S): Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0. 005). This was due to increase in both the cell count of ICM (P<0. 05) and TE (P<0.001). Coculture reduced the incidence of apoptosis in the cultured morula and blastocyst from 38% and 48% to 16% (P<0. 001) and 27% (P<0.05), respectively. The number of apoptotic blastomeres per morula (1.5 +/- 0.6; P<0.005) and blastocyst (2.3 +/- 0.7; P<0.005) after coculture was also significantly lower than that of the corresponding control (morula, 2.1 +/- 0.8; blastocyst, 3.5 +/- 1.1). CONCLUSION(S): Human oviductal cells improved mouse embryo development partly by decreasing the incidence of apoptosis.
OBJECTIVE: To investigate the effect of human oviductal cell coculture on the incidence of apoptosis in mouse embryos. DESIGN: Experimental laboratory study. SETTING: University gynecology unit. PATIENT(S): Fallopian tubes were obtained from patients undergoing hysterectomy. INTERVENTION(S): Mouse embryos were cocultured with human oviductal cells. MAIN OUTCOME MEASURE(S): Blastocyst development, allocation of inner cell mass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos. RESULT(S): Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0. 005). This was due to increase in both the cell count of ICM (P<0. 05) and TE (P<0.001). Coculture reduced the incidence of apoptosis in the cultured morula and blastocyst from 38% and 48% to 16% (P<0. 001) and 27% (P<0.05), respectively. The number of apoptotic blastomeres per morula (1.5 +/- 0.6; P<0.005) and blastocyst (2.3 +/- 0.7; P<0.005) after coculture was also significantly lower than that of the corresponding control (morula, 2.1 +/- 0.8; blastocyst, 3.5 +/- 1.1). CONCLUSION(S): Human oviductal cells improved mouse embryo development partly by decreasing the incidence of apoptosis.
Authors: Prasenjit Sarkar; Shan M Randall; Timothy S Collier; Anthony Nero; Teal A Russell; David C Muddiman; Balaji M Rao Journal: J Biol Chem Date: 2015-02-10 Impact factor: 5.157