Literature DB >> 11119561

Cytosolic delivery and characterization of the TcdB glucosylating domain by using a heterologous protein fusion.

L M Spyres1, M Qa'Dan, A Meader, J J Tomasek, E W Howard, J D Ballard.   

Abstract

TcdB from Clostridium difficile glucosylates small GTPases (Rho, Rac, and Cdc42) and is an important virulence factor in the human disease pseudomembranous colitis. In these experiments, in-frame genetic fusions between the genes for the 255 amino-terminal residues of anthrax toxin lethal factor (LFn) and the TcdB(1-556) coding region were constructed, expressed, and purified from Escherichia coli. LFnTcdB(1-556) was enzymatically active and glucosylated recombinant RhoA, Rac, Cdc42, and substrates from cell extracts. LFnTcdB(1-556) plus anthrax toxin protective antigen intoxicated cultured mammalian cells and caused actin reorganization and mouse lethality, all similar to those caused by wild-type TcdB.

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Year:  2001        PMID： 11119561      PMCID： PMC97927          DOI： 10.1128/IAI.69.1.599-601.2001

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  9 in total

1.  Comparative study of immunological properties and cytotoxic effects of Clostridium difficile toxin B and Clostridium sordellii toxin L.

Authors:  O Baldacini; R Girardot; G A Green; B Rihn; H Monteil
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Review 2.  Rho proteins: targets for bacterial toxins.

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4.  Chimeric clostridial cytotoxins: identification of the N-terminal region involved in protein substrate recognition.

Authors:  F Hofmann; C Busch; K Aktories
Journal:  Infect Immun       Date:  1998-03       Impact factor: 3.441

5.  Purification of two high molecular weight toxins of Clostridium difficile which are antigenically related.

Authors:  C von Eichel-Streiber; U Harperath; D Bosse; U Hadding
Journal:  Microb Pathog       Date:  1987-05       Impact factor: 3.738

6.  Protective antigen-binding domain of anthrax lethal factor mediates translocation of a heterologous protein fused to its amino- or carboxy-terminus.

Authors:  J C Milne; S R Blanke; P C Hanna; R J Collier
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7.  Clostridium difficile toxin B acts on the GTP-binding protein Rho.

Authors:  I Just; G Fritz; K Aktories; M Giry; M R Popoff; P Boquet; S Hegenbarth; C von Eichel-Streiber
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8.  Closing in on the toxic domain through analysis of a variant Clostridium difficile cytotoxin B.

Authors:  C von Eichel-Streiber; D Meyer zu Heringdorf; E Habermann; S Sartingen
Journal:  Mol Microbiol       Date:  1995-07       Impact factor: 3.501

9.  Residues 1-254 of anthrax toxin lethal factor are sufficient to cause cellular uptake of fused polypeptides.

Authors:  N Arora; S H Leppla
Journal:  J Biol Chem       Date:  1993-02-15       Impact factor: 5.486
  9 in total
  17 in total

1.  Mutational analysis of the enzymatic domain of Clostridium difficile toxin B reveals novel inhibitors of the wild-type toxin.

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4.  Inactivation of small Rho GTPases by the multifunctional RTX toxin from Vibrio cholerae.

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Journal:  Cell Microbiol       Date:  2007-05       Impact factor: 3.715

5.  Identification of a conserved membrane localization domain within numerous large bacterial protein toxins.

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Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-08       Impact factor: 11.205

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Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-11       Impact factor: 11.205

7.  Actin Crosslinking Toxins of Gram-Negative Bacteria.

Authors:  Karla J F Satchell
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8.  Variations in TcdB activity and the hypervirulence of emerging strains of Clostridium difficile.

Authors:  Jordi M Lanis; Soumitra Barua; Jimmy D Ballard
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Journal:  Infect Immun       Date:  2004-06       Impact factor: 3.441

10.  Identification of a His-Asp-Cys catalytic triad essential for function of the Rho inactivation domain (RID) of Vibrio cholerae MARTX toxin.

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Journal:  J Biol Chem       Date:  2012-11-26       Impact factor: 5.157
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