M Szatkowski1, M Mycielska, R Knowles, A L Kho, M B Djamgoz. 1. Cellular and Integrative Biology, Division of Biomedical Sciences and Department of Biology, Sir Alexander Fleming Building, Imperial College of Science, Technology and Medicine, London, UK. m.szatkowski@ic.ac.uk
Abstract
OBJECTIVE: To develop a preparation for the in vitro maintenance of the rat prostate gland and thus allow intracellular and transepithelial voltage measurements. MATERIALS AND METHODS: Ventral prostate glands from male rats were dissected free of connective tissue, separated into smaller lobes and maintained in vitro at 30 degrees C. Voltages were recorded with sharp micropipettes in identified cellular and luminal compartments, differentiated by several electrophysiological and histological parameters, including intracellular staining. RESULTS: Intracellular epithelial membrane potentials (median -40 mV) and transepithelial or luminal potentials (mean -4.2 mV) were recorded successfully. Luminal epithelial cells were dye-coupled. Prostate tissue could be maintained in vitro with no apparent electrophysiological or structural deterioration for up to approximately 7 h. CONCLUSION: Rat prostate tissue can be successfully maintained in vitro and electrophysiological recordings made from identified cellular compartments.
OBJECTIVE: To develop a preparation for the in vitro maintenance of the rat prostate gland and thus allow intracellular and transepithelial voltage measurements. MATERIALS AND METHODS: Ventral prostate glands from male rats were dissected free of connective tissue, separated into smaller lobes and maintained in vitro at 30 degrees C. Voltages were recorded with sharp micropipettes in identified cellular and luminal compartments, differentiated by several electrophysiological and histological parameters, including intracellular staining. RESULTS: Intracellular epithelial membrane potentials (median -40 mV) and transepithelial or luminal potentials (mean -4.2 mV) were recorded successfully. Luminal epithelial cells were dye-coupled. Prostate tissue could be maintained in vitro with no apparent electrophysiological or structural deterioration for up to approximately 7 h. CONCLUSION:Rat prostate tissue can be successfully maintained in vitro and electrophysiological recordings made from identified cellular compartments.
Authors: Cristina Martin-Granados; Alan R Prescott; Nele Van Dessel; Aleyde Van Eynde; Miguel Arocena; Izabela P Klaska; Janina Görnemann; Monique Beullens; Mathieu Bollen; John V Forrester; Colin D McCaig Journal: PLoS One Date: 2012-07-16 Impact factor: 3.240