Literature DB >> 11115754

Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks.

F Hayes1, B Hallet.   

Abstract

Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.

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Year:  2000        PMID： 11115754     DOI： 10.1016/s0966-842x(00)01857-6

Source DB:  PubMed          Journal:  Trends Microbiol        ISSN： 0966-842X            Impact factor:   17.079


  17 in total

1.  Identification of XcpZ domains required for assembly of the secreton of Pseudomonas aeruginosa.

Authors:  Viviane Robert; Finbarr Hayes; Andrée Lazdunski; Gérard P F Michel
Journal:  J Bacteriol       Date:  2002-03       Impact factor: 3.490

2.  Structure-function studies of Escherichia coli RpoH (sigma32) by in vitro linker insertion mutagenesis.

Authors:  Franz Narberhaus; Sylvia Balsiger
Journal:  J Bacteriol       Date:  2003-05       Impact factor: 3.490

3.  Expression of Helicobacter pylori vacuolating toxin in Escherichia coli.

Authors:  Mark S McClain; Timothy L Cover
Journal:  Infect Immun       Date:  2003-04       Impact factor: 3.441

4.  The Tra domain of the lactococcal CluA surface protein is a unique domain that contributes to sex factor DNA transfer.

Authors:  Régis Stentz; Mike Gasson; Claire Shearman
Journal:  J Bacteriol       Date:  2006-03       Impact factor: 3.490

5.  Site-specific incorporation of allosteric-inhibition sites in a protein tyrosine phosphatase.

Authors:  Xin-Yu Zhang; Anthony C Bishop
Journal:  J Am Chem Soc       Date:  2007-03-09       Impact factor: 15.419

6.  Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage mu.

Authors:  Arja Lamberg; Sari Nieminen; Mingqiang Qiao; Harri Savilahti
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

7.  The histidine kinase PdhS controls cell cycle progression of the pathogenic alphaproteobacterium Brucella abortus.

Authors:  Charles Van der Henst; François Beaufay; Johann Mignolet; Christian Didembourg; Julien Colinet; Bernard Hallet; Jean-Jacques Letesson; Xavier De Bolle
Journal:  J Bacteriol       Date:  2012-07-27       Impact factor: 3.490

8.  Identification of regions critically affecting kinetics and allosteric regulation of the Escherichia coli ADP-glucose pyrophosphorylase by modeling and pentapeptide-scanning mutagenesis.

Authors:  Miguel A Ballicora; Esteban D Erben; Terutaka Yazaki; Ana L Bertolo; Ana M Demonte; Jennifer R Schmidt; Mabel Aleanzi; Clarisa M Bejar; Carlos M Figueroa; Corina M Fusari; Alberto A Iglesias; Jack Preiss
Journal:  J Bacteriol       Date:  2007-05-11       Impact factor: 3.490

9.  Functional characterization of a portion of the Moloney murine leukemia virus gag gene by genetic footprinting.

Authors:  Marcy R Auerbach; Chang Shu; Artem Kaplan; Ila R Singh
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-22       Impact factor: 11.205

10.  Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis.

Authors:  Javier Sanchez Garcia; Andrey G Baranovskiy; Elena V Knatko; Fiona C Gray; Tahir H Tahirov; Stuart A MacNeill
Journal:  BMC Mol Biol       Date:  2009-08-17       Impact factor: 2.946
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