Literature DB >> 11115703

Influence of codon usage on the immunogenicity of a DNA vaccine against tetanus.

R Stratford1, G Douce, L Zhang-Barber, N Fairweather, J Eskola, G Dougan.   

Abstract

Two related DNA vaccine vector plasmids, harbouring either wild-type (pcDNA3/ntetC) or synthetic codon optimised (pcDNA3/stetC) DNA encoding fragment C (TetC) of tetanus toxin were constructed. COS-7 cells transformed with pcDNA3/stetC reproducibly expressed higher levels of TetC than similar cells transformed with pcDNA3/ntetC. BALB/c mice immunised intramuscularly with pcDNA3/stetC produced significantly higher levels of anti-TetC antibodies in their serum in the weeks following vaccination compared to mice immunised with pcDNA3/ntetC, even when differences in the CpG content between the two sequences were controlled for using non-expressing DNA.

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Year:  2000        PMID： 11115703     DOI： 10.1016/s0264-410x(00)00246-2

Source DB:  PubMed          Journal:  Vaccine        ISSN： 0264-410X            Impact factor:   3.641


  20 in total

1.  Expression of tetanus toxin Fragment C in tobacco chloroplasts.

Authors:  John S Tregoning; Peter Nixon; Hiroshi Kuroda; Zora Svab; Simon Clare; Frances Bowe; Neil Fairweather; Jimmy Ytterberg; Klaas J van Wijk; Gordon Dougan; Pal Maliga
Journal:  Nucleic Acids Res       Date:  2003-02-15       Impact factor: 16.971

2.  Optimization of codon usage enhances the immunogenicity of a DNA vaccine encoding mycobacterial antigen Ag85B.

Authors:  Hyun-Jeong Ko; Sung-Youl Ko; Yeon-Jeong Kim; Eun-Gae Lee; Sang-Nae Cho; Chang-Yuil Kang
Journal:  Infect Immun       Date:  2005-09       Impact factor: 3.441

3.  Alphavirus replicon approach to promoterless analysis of IRES elements.

Authors:  K I Kamrud; M Custer; J M Dudek; G Owens; K D Alterson; J S Lee; J L Groebner; J F Smith
Journal:  Virology       Date:  2006-12-06       Impact factor: 3.616

4.  Hemagglutinin (HA) proteins from H1 and H3 serotypes of influenza A viruses require different antigen designs for the induction of optimal protective antibody responses as studied by codon-optimized HA DNA vaccines.

Authors:  Shixia Wang; Jessica Taaffe; Christopher Parker; Alicia Solórzano; Hong Cao; Adolfo García-Sastre; Shan Lu
Journal:  J Virol       Date:  2006-09-20       Impact factor: 5.103

5.  Generation of high-titer neutralizing antibodies against botulinum toxins A, B, and E by DNA electrotransfer.

Authors:  C Trollet; Y Pereira; A Burgain; E Litzler; M Mezrahi; J Seguin; M Manich; M R Popoff; D Scherman; P Bigey
Journal:  Infect Immun       Date:  2009-02-23       Impact factor: 3.441

Review 6.  DNA vaccines for targeting bacterial infections.

Authors:  Mariana Ingolotti; Omkar Kawalekar; Devon J Shedlock; Karuppiah Muthumani; David B Weiner
Journal:  Expert Rev Vaccines       Date:  2010-07       Impact factor: 5.217

7.  Mycobacterial codon optimization enhances antigen expression and virus-specific immune responses in recombinant Mycobacterium bovis bacille Calmette-Guérin expressing human immunodeficiency virus type 1 Gag.

Authors:  Masaru Kanekiyo; Kazuhiro Matsuo; Makiko Hamatake; Takaichi Hamano; Takeaki Ohsu; Sohkichi Matsumoto; Takeshi Yamada; Shudo Yamazaki; Atsuhiko Hasegawa; Naoki Yamamoto; Mitsuo Honda
Journal:  J Virol       Date:  2005-07       Impact factor: 5.103

8.  Antigen engineering can play a critical role in the protective immunity elicited by Yersinia pestis DNA vaccines.

Authors:  Shixia Wang; Innocent Mboudjeka; Jon D Goguen; Shan Lu
Journal:  Vaccine       Date:  2010-02-23       Impact factor: 3.641

9.  Intratumoral DNA electroporation induces anti-tumor immunity and tumor regression.

Authors:  Olga Radkevich-Brown; Marie P Piechocki; Jessica B Back; Amy M Weise; Shari Pilon-Thomas; Wei-Zen Wei
Journal:  Cancer Immunol Immunother       Date:  2010-03       Impact factor: 6.968

10.  Enhancement of the expression of HCV core gene does not enhance core-specific immune response in DNA immunization: advantages of the heterologous DNA prime, protein boost immunization regimen.

Authors:  Ekaterina Alekseeva; Irina Sominskaya; Dace Skrastina; Irina Egorova; Elizaveta Starodubova; Eriks Kushners; Marija Mihailova; Natalia Petrakova; Ruta Bruvere; Tatyana Kozlovskaya; Maria Isaguliants; Paul Pumpens
Journal:  Genet Vaccines Ther       Date:  2009-06-08
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