Literature DB >> 11114939

Quorum sensing in Vibrio fischeri: analysis of the LuxR DNA binding region by alanine-scanning mutagenesis.

K A Egland1, E P Greenberg.   

Abstract

LuxR is the transcriptional activator for quorum-sensing control of luminescence in Vibrio fischeri. A series of alanine-scanning mutants spanning a predicted helix-turn-helix region in the DNA binding domain of LuxR was constructed, and the activity of each of the LuxR mutant proteins in recombinant Escherichia coli was investigated. The region covered by the mutagenesis spanned residues 190 to 224. About half of the alanine-scanning mutants showed activities similar to that of the wild-type LuxR: at least two were positive-control mutants, four appeared to be defective in DNA binding, and several others were characterized as DNA binding affinity mutants. This analysis, taken together with information about other bacterial transcription factors, provides insights into amino acid residues in LuxR that are involved in DNA binding and transcriptional activation.

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Year:  2001        PMID： 11114939      PMCID： PMC94888          DOI： 10.1128/JB.183.1.382-386.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  29 in total

1.  Signal-dependent DNA binding and functional domains of the quorum-sensing activator TraR as identified by repressor activity.

Authors:  Z Q Luo; S K Farrand
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

2.  Overproduction and purification of the luxR gene product: Transcriptional activator of the Vibrio fischeri luminescence system.

Authors:  H B Kaplan; E P Greenberg
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

Review 3.  Census and consensus in bacterial ecosystems: the LuxR-LuxI family of quorum-sensing transcriptional regulators.

Authors:  C Fuqua; S C Winans; E P Greenberg
Journal:  Annu Rev Microbiol       Date:  1996       Impact factor: 15.500

4.  Quorum sensing in Vibrio fischeri: essential elements for activation of the luminescence genes.

Authors:  A M Stevens; E P Greenberg
Journal:  J Bacteriol       Date:  1997-01       Impact factor: 3.490

5.  Structure of the Escherichia coli response regulator NarL.

Authors:  I Baikalov; I Schröder; M Kaczor-Grzeskowiak; K Grzeskowiak; R P Gunsalus; R E Dickerson
Journal:  Biochemistry       Date:  1996-08-27       Impact factor: 3.162

6.  Critical regions of the Vibrio fischeri luxR protein defined by mutational analysis.

Authors:  J Slock; D VanRiet; D Kolibachuk; E P Greenberg
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

7.  Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers.

Authors:  Y Zhou; S Busby; R H Ebright
Journal:  Cell       Date:  1993-04-23       Impact factor: 41.582

Review 8.  Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators.

Authors:  W C Fuqua; S C Winans; E P Greenberg
Journal:  J Bacteriol       Date:  1994-01       Impact factor: 3.490

9.  Identification of genes and gene products necessary for bacterial bioluminescence.

Authors:  J Engebrecht; M Silverman
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

10.  Control of Vibrio fischeri luminescence gene expression in Escherichia coli by cyclic AMP and cyclic AMP receptor protein.

Authors:  P V Dunlap; E P Greenberg
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490
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  29 in total

1.  Identification of a DNA binding region in GerE from Bacillus subtilis.

Authors:  D L Crater; C P Moran
Journal:  J Bacteriol       Date:  2001-07       Impact factor: 3.490

2.  The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA.

Authors:  Alessandro Vannini; Cinzia Volpari; Cesare Gargioli; Ester Muraglia; Riccardo Cortese; Raffaele De Francesco; Petra Neddermann; Stefania Di Marco
Journal:  EMBO J       Date:  2002-09-02       Impact factor: 11.598

3.  Directed evolution of Vibrio fischeri LuxR for improved response to butanoyl-homoserine lactone.

Authors:  Andrew C Hawkins; Frances H Arnold; Rainer Stuermer; Bernhard Hauer; Jared R Leadbetter
Journal:  Appl Environ Microbiol       Date:  2007-08-03       Impact factor: 4.792

Review 4.  Decoding microbial chatter: cell-cell communication in bacteria.

Authors:  Karen L Visick; Clay Fuqua
Journal:  J Bacteriol       Date:  2005-08       Impact factor: 3.490

5.  Implications of rewiring bacterial quorum sensing.

Authors:  Eric L Haseltine; Frances H Arnold
Journal:  Appl Environ Microbiol       Date:  2007-11-26       Impact factor: 4.792

6.  proTeOn and proTeOff, new protein devices that inducibly activate bacterial gene expression.

Authors:  Katherine Volzing; Konstantinos Biliouris; Yiannis N Kaznessis
Journal:  ACS Chem Biol       Date:  2011-08-18       Impact factor: 5.100

Review 7.  LuxR-type quorum-sensing regulators that are detached from common scents.

Authors:  Ching-Sung Tsai; Stephen C Winans
Journal:  Mol Microbiol       Date:  2010-09       Impact factor: 3.501

8.  Inhibitory Effect of Taurine on Biofilm Formation During Alkane Degradation in Acinetobacter oleivorans DR1.

Authors:  Hyo Jung Eom; Woojun Park
Journal:  Microb Ecol       Date:  2017-06-15       Impact factor: 4.552

9.  Identification of amino acid residues of the pheromone-binding domain of the transcription factor TraR that are required for positive control.

Authors:  Esther D Costa; Hongbaek Cho; Stephen C Winans
Journal:  Mol Microbiol       Date:  2009-07-06       Impact factor: 3.501

10.  Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.

Authors:  M L Urbanowski; C P Lostroh; E P Greenberg
Journal:  J Bacteriol       Date:  2004-02       Impact factor: 3.490
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