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Literature DB >> 111144

Sequences at the somatic recombination sites of immunoglobulin light-chain genes.

H Sakano, K Hüppi, G Heinrich, S Tonegawa.

Abstract

The entire nucleotide sequence of a 1.7-kilobase embryonic DNA fragment containing five joining (J) DNA segments for mouse immunoglobulin kappa chain gene has been determined. Each J DNA segment can encode amino acid residues 96--108. Comparison of one of the five J DNA sequences with those of an embryonic variable (V) gene and a complete kappa chain gene permitted localisation of a precise recombination site. The 5'-flanking regions of J DNA segments could form an inverted stem structure with the 3'-non-coding region of embryonic V genes. This hypothetical structure and gel-blotting analysis of total embryo and myeloma DNA suggest that the somatic recombination may be accompanied by excision of an entire DNA segment between a V gene and a J DNA segment. Antibody diversity may in part be generated by modulation of the precise recombination sites.

Entities: Disease Gene Species

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Year: 1979 PMID： 111144 DOI： 10.1038/280288a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

308 in total

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3. Regulation of RAG1/RAG2-mediated transposition by GTP and the C-terminal region of RAG2.

Authors: Chia-Lun Tsai; David G Schatz
Journal: EMBO J Date: 2003-04-15 Impact factor: 11.598

4. B-cell proliferation initiated by Ia cross-linking and sustained by interleukins leads to class switching but not somatic mutation in vitro.

Authors: L J Wysocki; G Creadon; K R Lehmann; J C Cambier
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10. Diversity at the variable-joining region boundary of lambda light chains has a pronounced effect on immunoglobulin ligand-binding activity.

Authors: T Azuma; V Igras; E B Reilly; H N Eisen
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

Sequences at the somatic recombination sites of immunoglobulin light-chain genes.

Review 1. Transposition mediated by RAG1 and RAG2 and the evolution of the adaptive immune system.

2. RAG transposase can capture and commit to target DNA before or after donor cleavage.

3. Regulation of RAG1/RAG2-mediated transposition by GTP and the C-terminal region of RAG2.

4. B-cell proliferation initiated by Ia cross-linking and sustained by interleukins leads to class switching but not somatic mutation in vitro.

5. In vitro processing of the 3'-overhanging DNA in the postcleavage complex involved in V(D)J joining.

6. Ordered DNA release and target capture in RAG transposition.

Review 7. Evolutionary consequences of nonrandom damage and repair of chromatin domains.

Review 8. Role of recombination activating genes in the generation of antigen receptor diversity and beyond.

Review 9. A cold-blooded view of adaptive immunity.

10. Diversity at the variable-joining region boundary of lambda light chains has a pronounced effect on immunoglobulin ligand-binding activity.