Literature DB >> 11112512

Expression of a bispecific dsFv-dsFv' antibody fragment in Escherichia coli.

A Schmiedl1, F Breitling, S Dübel.   

Abstract

A bispecific disulfide-stabilized Fv antibody fragment (dsFv-dsFv') consisting of two different disulfide-stabilized Fv antibody fragments connected by flexible linker peptides was produced by secretion of three polypeptide chains into the periplasm of Escherichia coli. The dsFv-dsFv' molecules were enriched by immobilized metal affinity chromatography and further purified by anion-exchange chromatography. The recombinant antibody constructs retained the two parental antigen binding specificities and were able to cross-link the two different antigens. The described dsFv-dsFv' design might be of particular value for therapeutic in vivo applications since improved stability is expected to be combined with minimal immunogenicity.

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Year:  2000        PMID： 11112512     DOI： 10.1093/protein/13.10.725

Source DB:  PubMed          Journal:  Protein Eng        ISSN： 0269-2139


  14 in total

1.  Stably tethered multifunctional structures of defined composition made by the dock and lock method for use in cancer targeting.

Authors:  Edmund A Rossi; David M Goldenberg; Thomas M Cardillo; William J McBride; Robert M Sharkey; Chien-Hsing Chang
Journal:  Proc Natl Acad Sci U S A       Date:  2006-04-24       Impact factor: 11.205

Review 2.  Generation and production of engineered antibodies.

Authors:  Sergey M Kipriyanov; Fabrice Le Gall
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

3.  Selection for Anti-transferrin Receptor Bispecific T-cell Engager in Different Molecular Formats.

Authors:  Ming-Peng Fu; Zi-Long Guo; Hong-Ling Tang; Hui-Fen Zhu; Guan-Xin Shen; Yong He; Ping Lei
Journal:  Curr Med Sci       Date:  2020-03-13

4.  Bispecific antibody derivatives with restricted binding functionalities that are activated by proteolytic processing.

Authors:  Silke Metz; Christian Panke; Alexander K Haas; Jürgen Schanzer; Wilma Lau; Rebecca Croasdale; Eike Hoffmann; Britta Schneider; Johannes Auer; Christian Gassner; Birgit Bossenmaier; Pablo Umana; Claudio Sustmann; Ulrich Brinkmann
Journal:  Protein Eng Des Sel       Date:  2012-09-13       Impact factor: 1.650

5.  Production of recombinant antibody fragments in Bacillus megaterium.

Authors:  Eva Jordan; Michael Hust; Andreas Roth; Rebekka Biedendieck; Thomas Schirrmann; Dieter Jahn; Stefan Dübel
Journal:  Microb Cell Fact       Date:  2007-01-15       Impact factor: 5.328

Review 6.  The making of bispecific antibodies.

Authors:  Ulrich Brinkmann; Roland E Kontermann
Journal:  MAbs       Date:  2017 Feb/Mar       Impact factor: 5.857

7.  Characterization and analysis of scFv-IgG bispecific antibody size variants.

Authors:  Mingyan Cao; Chunlei Wang; Wai Keen Chung; Dana Motabar; Jihong Wang; Elizabeth Christian; Shihua Lin; Alan Hunter; Xiangyang Wang; Dengfeng Liu
Journal:  MAbs       Date:  2018-09-20       Impact factor: 5.857

8.  Production of single chain Fab (scFab) fragments in Bacillus megaterium.

Authors:  Eva Jordan; Laila Al-Halabi; Thomas Schirrmann; Michael Hust; Stefan Dübel
Journal:  Microb Cell Fact       Date:  2007-11-27       Impact factor: 5.328

9.  Single chain Fab (scFab) fragment.

Authors:  Michael Hust; Thomas Jostock; Christian Menzel; Bernd Voedisch; Anja Mohr; Mariam Brenneis; Martina I Kirsch; Doris Meier; Stefan Dübel
Journal:  BMC Biotechnol       Date:  2007-03-08       Impact factor: 2.563

10.  Functional comparison of single-chain and two-chain anti-CD3-based bispecific antibodies in gene immunotherapy applications.

Authors:  Marta Compte; Ana Alvarez-Cienfuegos; Natalia Nuñez-Prado; Noelia Sainz-Pastor; Ana Blanco-Toribio; Nuria Pescador; Laura Sanz; Luis Alvarez-Vallina
Journal:  Oncoimmunology       Date:  2014-05-23       Impact factor: 8.110
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