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Literature DB >> 11108727

A simple and highly sensitive radioenzymatic assay for lysophosphatidic acid quantification.

J S Saulnier-Blache¹, A Girard, M F Simon, M Lafontan, P Valet.

Abstract

The objective of the present work was to develop a simple and sensitive radioenzymatic assay to quantify lysophosphatidic acid (LPA). For that, a recombinant rat LPA acid acyltransferase (LPAAT) produced in Escherichia coli was used. In the presence of [(14)C]oleoyl-CoA, LPAAT selectively catalyzes the transformation of LPA and alkyl-LPA into [(14)C]phosphatidic acid. Acylation of LPA was complete and linear from 0 to 200 pmol with a minimal detection of 0.2 pmol. This method was used to quantify LPA in butanol-extracted lipids from bovine sera, as well as from human and mouse plasma. This radioenzymatic assay represents a new, simple, and highly sensitive method to quantify LPA in various biological fluids.

Entities: Chemical Gene Species

Mesh：

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Year: 2000 PMID： 11108727 PMCID： PMC2020493

Source DB: PubMed Journal: J Lipid Res ISSN： 0022-2275 Impact factor: 5.922

27 in total

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29 in total

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