BACKGROUND: Ras protein is a key signal transducer in the cause of cell proliferation. We studied the effects of active and negative mutants of the Ras gene on arterial neointimal formation in rats, with the aim of elucidating the molecular mechanisms regulating restenosis following percutaneous transluminal coronary angioplasty. MATERIALS AND METHODS: AdRasV12 and AdRasN17, the recombinant adenoviruses containing a constitutively active mutant and a dominant negative mutant of Ras, respectively, were used to determine whether Ras is necessary and sufficient to modulate the smooth muscle cell proliferation and neointima formation. Following balloon injury, rat common carotid arteries were treated in their distal half with AdRasV12, AdRasN17, or AdLacZ, with the proximal half used as uninfected control. RESULTS: In rat arteries subjected to balloon injury, either uninfected or treated with AdLacZ, there were pronounced SMC proliferation and neointima formation. These changes were markedly augmented by AdRasV12 and reduced by AdRasN17. CONCLUSION: Ras is necessary and sufficient for SMC proliferation and neointima formation and may play a critical role in restenosis following balloon angioplasty. Copyright 2000 Academic Press.
BACKGROUND: Ras protein is a key signal transducer in the cause of cell proliferation. We studied the effects of active and negative mutants of the Ras gene on arterial neointimal formation in rats, with the aim of elucidating the molecular mechanisms regulating restenosis following percutaneous transluminal coronary angioplasty. MATERIALS AND METHODS: AdRasV12 and AdRasN17, the recombinant adenoviruses containing a constitutively active mutant and a dominant negative mutant of Ras, respectively, were used to determine whether Ras is necessary and sufficient to modulate the smooth muscle cell proliferation and neointima formation. Following balloon injury, rat common carotid arteries were treated in their distal half with AdRasV12, AdRasN17, or AdLacZ, with the proximal half used as uninfected control. RESULTS: In rat arteries subjected to balloon injury, either uninfected or treated with AdLacZ, there were pronounced SMC proliferation and neointima formation. These changes were markedly augmented by AdRasV12 and reduced by AdRasN17. CONCLUSION: Ras is necessary and sufficient for SMC proliferation and neointima formation and may play a critical role in restenosis following balloon angioplasty. Copyright 2000 Academic Press.
Authors: Brian K Stansfield; Waylan K Bessler; Raghuveer Mali; Julie A Mund; Brandon D Downing; Reuben Kapur; David A Ingram Journal: Am J Pathol Date: 2013-11-07 Impact factor: 4.307
Authors: Elisabeth A Lasater; Waylan K Bessler; Laura E Mead; Whitney E Horn; D Wade Clapp; Simon J Conway; David A Ingram; Fang Li Journal: Hum Mol Genet Date: 2008-04-28 Impact factor: 6.150
Authors: Paula J Klutho; Steven M Pennington; Jason A Scott; Katina M Wilson; Sean X Gu; Prakash Doddapattar; Litao Xie; Ashlee N Venema; Linda J Zhu; Anil K Chauhan; Steven R Lentz; Isabella M Grumbach Journal: Arterioscler Thromb Vasc Biol Date: 2015-10-08 Impact factor: 8.311
Authors: Brian K Stansfield; Waylan K Bessler; Raghuveer Mali; Julie A Mund; Brandon Downing; Fang Li; Kara N Sarchet; Matthew R DiStasi; Simon J Conway; Reuben Kapur; David A Ingram Journal: Hum Mol Genet Date: 2012-11-29 Impact factor: 6.150