Literature DB >> 11104003

Protein production: feeding the crystallographers and NMR spectroscopists.

A M Edwards1, C H Arrowsmith, D Christendat, A Dharamsi, J D Friesen, J F Greenblatt, M Vedadi.   

Abstract

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

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Year:  2000        PMID: 11104003     DOI: 10.1038/80751

Source DB:  PubMed          Journal:  Nat Struct Biol        ISSN: 1072-8368


  38 in total

1.  Determination of domain structure of proteins from X-ray solution scattering.

Authors:  D I Svergun; M V Petoukhov; M H Koch
Journal:  Biophys J       Date:  2001-06       Impact factor: 4.033

2.  Characterization of Ad5 E3-14.7K, an adenoviral inhibitor of apoptosis: structure, oligomeric state, and metal binding.

Authors:  Hee-Jung Kim; Mark P Foster
Journal:  Protein Sci       Date:  2002-05       Impact factor: 6.725

Review 3.  Utilizing NMR to study the structure of growth-inhibitory proteins.

Authors:  Francesca Marassi
Journal:  Methods Mol Biol       Date:  2003

4.  Role for NMR in structural genomics.

Authors:  Michael A Kennedy; Gaetano T Montelione; Cheryl H Arrowsmith; John L Markley
Journal:  J Struct Funct Genomics       Date:  2002

5.  Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins.

Authors:  Karen M Routzahn; David S Waugh
Journal:  J Struct Funct Genomics       Date:  2002

6.  Addition of missing loops and domains to protein models by x-ray solution scattering.

Authors:  Maxim V Petoukhov; Nigel A J Eady; Katherine A Brown; Dmitri I Svergun
Journal:  Biophys J       Date:  2002-12       Impact factor: 4.033

7.  Rapid purification and crystal structure analysis of a small protein carrying two terminal affinity tags.

Authors:  Uwe Mueller; Konrad Büssow; Anne Diehl; Franz J Bartl; Frank H Niesen; Lajos Nyarsik; Udo Heinemann
Journal:  J Struct Funct Genomics       Date:  2003

8.  Expression screening, protein purification and NMR analysis of human protein domains for structural genomics.

Authors:  G E Folkers; B N M van Buuren; R Kaptein
Journal:  J Struct Funct Genomics       Date:  2004

9.  Expression of human proteins at the Southeast Collaboratory for Structural Genomics.

Authors:  Michael R Mayer; Tamara A Dailey; Clay M Baucom; Jill L Supernak; Michael C Grady; Harris E Hawk; Harry A Dailey
Journal:  J Struct Funct Genomics       Date:  2004

Review 10.  Recombinant protein expression in Leishmania tarentolae.

Authors:  Giancarlo Basile; Manuela Peticca
Journal:  Mol Biotechnol       Date:  2009-11       Impact factor: 2.695

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