| Literature DB >> 11098091 |
K T Papazisis1, D Zambouli, O T Kimoundri, E S Papadakis, V Vala, G D Geromichalos, S Voyatzi, D Markala, E Destouni, L Boutis, A H Kortsaris.
Abstract
Genistein, is a natural isoflavone compound with a potent activity against protein tyrosine kinases. The leukemic cell line, K562, is a bcr/abl (Philadelphia chromosome) positive cell line that is resistant to DNA-damaging agents, including gamma-irradiation. Treatment with genistein increased apoptosis and promoted G2-phase arrest in the non-apoptotic population of the gamma-irradiated K562 cells. Irradiated cells that survived 72 h after the irradiation had a normal distribution in cell cycle, whilst genistein treatment kept cells arrested in the G2-phase, decreased the S-phase fraction and suppressed DNA-synthesis. Taken together, our results show that genistein augments apoptotic cell death after gamma-irradiation in K562 cells and this result cannot be attributed to abrogation of the G2/M checkpoint.Entities:
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Year: 2000 PMID： 11098091 DOI： 10.1016/s0304-3835(00)00569-3
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679