Literature DB >> 11090193

Affinity-tagged miniprion derivatives spontaneously adopt protease-resistant conformations.

S Supattapone1, H O Nguyen, T Muramoto, F E Cohen, S J DeArmond, S B Prusiner, M Scott.   

Abstract

An abridged PrP molecule of 106 amino acids designated PrP106 can form infectious miniprions in transgenic (Tg) mice (29). Addition of six-histidine (His(6)) affinity tags to selective sites within PrP106 resulted unexpectedly in new PrP proteins that spontaneously adopted protease-resistant conformations when expressed in neuroblastoma cells and Tg mice. Acquisition of protease resistance depended on the length, charge, and placement of the affinity tag. Introduction of the disease-linked mutation E200K into the sequence of PrP106(140/6His) increased the recovery of protease-resistant PrP fivefold, whereas introduction of the mutations C213A and Delta214-220 did not affect the recovery of protease-resistant PrP. Treatment of cultured cells expressing affinity-tagged PrP106 mutants with polypropyleneimine dendrimer rendered these proteins sensitive to protease digestion in a manner similar to wild-type PrP(Sc). We conclude that certain affinity-tagged PrP106 proteins spontaneously fold into conformations partially resembling, yet distinct from, wild-type PrP(Sc). These proteins might be useful tools in the identification of new disease-causing mutations as well as for screening compounds for therapeutic efficacy.

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Year:  2000        PMID: 11090193      PMCID: PMC112476          DOI: 10.1128/jvi.74.24.11928-11934.2000

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  29 in total

Review 1.  Familial Creutzfeldt-Jakob disease. Codon 200 prion disease in Libyan Jews.

Authors:  Z Meiner; R Gabizon; S B Prusiner
Journal:  Medicine (Baltimore)       Date:  1997-07       Impact factor: 1.889

2.  Separation of scrapie prion infectivity from PrP amyloid polymers.

Authors:  H Wille; G F Zhang; M A Baldwin; F E Cohen; S B Prusiner
Journal:  J Mol Biol       Date:  1996-06-21       Impact factor: 5.469

3.  Autonomous and reversible folding of a soluble amino-terminally truncated segment of the mouse prion protein.

Authors:  S Hornemann; R Glockshuber
Journal:  J Mol Biol       Date:  1996-09-06       Impact factor: 5.469

4.  Recombinant scrapie-like prion protein of 106 amino acids is soluble.

Authors:  T Muramoto; M Scott; F E Cohen; S B Prusiner
Journal:  Proc Natl Acad Sci U S A       Date:  1996-12-24       Impact factor: 11.205

5.  Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD.

Authors:  J Collinge; K C Sidle; J Meads; J Ironside; A F Hill
Journal:  Nature       Date:  1996-10-24       Impact factor: 49.962

Review 6.  Inherited prion diseases.

Authors:  S B Prusiner
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205

7.  Conformational transitions, dissociation, and unfolding of scrapie amyloid (prion) protein.

Authors:  J Safar; P P Roller; D C Gajdusek; C J Gibbs
Journal:  J Biol Chem       Date:  1993-09-25       Impact factor: 5.157

8.  Recombinant full-length murine prion protein, mPrP(23-231): purification and spectroscopic characterization.

Authors:  S Hornemann; C Korth; B Oesch; R Riek; G Wider; K Wüthrich; R Glockshuber
Journal:  FEBS Lett       Date:  1997-08-18       Impact factor: 4.124

9.  Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity.

Authors:  G C Telling; P Parchi; S J DeArmond; P Cortelli; P Montagna; R Gabizon; J Mastrianni; E Lugaresi; P Gambetti; S B Prusiner
Journal:  Science       Date:  1996-12-20       Impact factor: 47.728

10.  Semipreparative chromatographic method to purify the normal cellular isoform of the prion protein in nondenatured form.

Authors:  P Pergami; H Jaffe; J Safar
Journal:  Anal Biochem       Date:  1996-04-05       Impact factor: 3.365

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  3 in total

1.  Metal-bound claMP Tag inhibits proteolytic cleavage.

Authors:  Michaela L McNiff; Jennifer S Chadwick
Journal:  Protein Eng Des Sel       Date:  2017-06-01       Impact factor: 1.650

2.  Generating Bona Fide Mammalian Prions with Internal Deletions.

Authors:  Carola Munoz-Montesino; Christina Sizun; Mohammed Moudjou; Laetitia Herzog; Fabienne Reine; Jérôme Chapuis; Danica Ciric; Angelique Igel-Egalon; Hubert Laude; Vincent Béringue; Human Rezaei; Michel Dron
Journal:  J Virol       Date:  2016-07-11       Impact factor: 5.103

3.  Molecular distinction between pathogenic and infectious properties of the prion protein.

Authors:  Roberto Chiesa; Pedro Piccardo; Elena Quaglio; Bettina Drisaldi; San Ling Si-Hoe; Masaki Takao; Bernardino Ghetti; David A Harris
Journal:  J Virol       Date:  2003-07       Impact factor: 5.103

  3 in total

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