PURPOSE: Irradiation inflicts acute injuries to the intestinal mucosa with rapid apoptosis induction and subsequent reduction in epithelial surface area. It may therefore be assumed that the intestinal barrier function is affected. The aim of this study was to compare the mucosal permeability in irradiated rectum and nonirradiated sigmoid colon from patients subjected to radiation therapy before surgical treatment for rectal cancer. METHODS: Segments from sigmoid colon and rectum obtained from irradiated and nonirradiated patients were stripped from the serosa-muscle layer and mounted in Ussing diffusion chambers. The mucosa-to-serosa passage of the marker molecules 14C-mannitol, fluorescein isothiocyanate-dextran 4,400, and ovalbumin was followed for 120 minutes. RESULTS: The permeability to the markers was size-dependent and increased linearly across time in all specimens. The passage of all markers was increased in irradiated rectum compared with nonirradiated sigmoid colon, whereas in specimens from nonirradiated patients there were no differences between rectum and sigmoid colon. Histologic signs of crypt and mucosal atrophy were found in the irradiated rectal specimens. CONCLUSIONS: Early gastrointestinal complications after radiation therapy may be the result of mucosal atrophy in addition to mucosal damage, with a loss of barrier integrity.
PURPOSE: Irradiation inflicts acute injuries to the intestinal mucosa with rapid apoptosis induction and subsequent reduction in epithelial surface area. It may therefore be assumed that the intestinal barrier function is affected. The aim of this study was to compare the mucosal permeability in irradiated rectum and nonirradiated sigmoid colon from patients subjected to radiation therapy before surgical treatment for rectal cancer. METHODS: Segments from sigmoid colon and rectum obtained from irradiated and nonirradiated patients were stripped from the serosa-muscle layer and mounted in Ussing diffusion chambers. The mucosa-to-serosa passage of the marker molecules 14C-mannitol, fluorescein isothiocyanate-dextran 4,400, and ovalbumin was followed for 120 minutes. RESULTS: The permeability to the markers was size-dependent and increased linearly across time in all specimens. The passage of all markers was increased in irradiated rectum compared with nonirradiated sigmoid colon, whereas in specimens from nonirradiated patients there were no differences between rectum and sigmoid colon. Histologic signs of crypt and mucosal atrophy were found in the irradiated rectal specimens. CONCLUSIONS: Early gastrointestinal complications after radiation therapy may be the result of mucosal atrophy in addition to mucosal damage, with a loss of barrier integrity.
Authors: Qiang Fu; Maaike Berbée; Wenze Wang; Marjan Boerma; Junru Wang; Herbert A Schmid; Martin Hauer-Jensen Journal: Radiat Res Date: 2011-04-29 Impact factor: 2.841
Authors: Maryam Goudarzi; Tytus D Mak; Jonathan P Jacobs; Bo-Hyun Moon; Steven J Strawn; Jonathan Braun; David J Brenner; Albert J Fornace; Heng-Hong Li Journal: Radiat Res Date: 2016-08-11 Impact factor: 2.841
Authors: Velda J Gonzalez-Mercado; Sara Marrero; Josué Pérez-Santiago; Maribel Tirado-Gómez; Miguel A Marrero-Falcón; Elsa Pedro; Leorey N Saligan Journal: P R Health Sci J Date: 2021-03 Impact factor: 0.600
Authors: Sendhilnathan Ramalingam; Sharareh Siamakpour-Reihani; Lauren Bohannan; Yi Ren; Alexander Sibley; Jeff Sheng; Li Ma; Andrew B Nixon; Jing Lyu; Daniel C Parker; James Bain; Michael Muehlbauer; Olga Ilkayeva; Virginia Byers Kraus; Janet L Huebner; Thomas Spitzer; Jami Brown; Jonathan U Peled; Marcel van den Brink; Antonio Gomes; Taewoong Choi; Cristina Gasparetto; Mitchell Horwitz; Gwynn Long; Richard Lopez; David Rizzieri; Stefanie Sarantopoulos; Nelson Chao; Anthony D Sung Journal: PLoS One Date: 2021-06-25 Impact factor: 3.240