| Literature DB >> 11087467 |
M Murata1, M Haruta, N Murai, N Tanikawa, M Nishimura, S Homma, Y Itoh.
Abstract
Transgenic apple shoots were prepared from leaf disks by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistance gene and antisense polyphenol oxidase (PPO) DNA. Four transgenic apple lines that grew on the medium containing 50 microgram/mL KM were obtained. They contained the KM resistance gene and grew stably on the medium for >3 years. Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot.Entities:
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Year: 2000 PMID： 11087467 DOI： 10.1021/jf000771m
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279