PURPOSE: Generation of valuable information about the biphasic geometrical configuration of porcine stratum corneum from Very Small Angle Neutron Scattering (VSANS) data and investigation of its effect on the corresponding effective diffusivity. METHODS: Spectra of porcine stratum corneum are mathematically transformed in order to obtain the corresponding auto-correlation function (ACF). Model stratum corneum structures, matching this experimentally determined ACF, are then produced based on the "brick-and-mortar" configuration. The effective diffusivity through these model domains is calculated using an appropriate numerical method. RESULTS: The most appropriate geometry of porcine stratum corneum's lipid and protein phases in a "brick-and-mortar" configuration is quantitatively determined and correlated with the barrier properties (diffusivity) of the stratum corneum model structures. CONCLUSIONS: The ACF analysis indicates the most appropriate values for the dimensions of the corneocyte thickness and the surrounding lipid gap, while the corneocyte length is estimated from the diffusion study.
PURPOSE: Generation of valuable information about the biphasic geometrical configuration of porcine stratum corneum from Very Small Angle Neutron Scattering (VSANS) data and investigation of its effect on the corresponding effective diffusivity. METHODS: Spectra of porcine stratum corneum are mathematically transformed in order to obtain the corresponding auto-correlation function (ACF). Model stratum corneum structures, matching this experimentally determined ACF, are then produced based on the "brick-and-mortar" configuration. The effective diffusivity through these model domains is calculated using an appropriate numerical method. RESULTS: The most appropriate geometry of porcine stratum corneum's lipid and protein phases in a "brick-and-mortar" configuration is quantitatively determined and correlated with the barrier properties (diffusivity) of the stratum corneum model structures. CONCLUSIONS: The ACF analysis indicates the most appropriate values for the dimensions of the corneocyte thickness and the surrounding lipid gap, while the corneocyte length is estimated from the diffusion study.
Authors: G C Charalambopoulou; T A Steriotis; A C Mitropoulos; K L Stefanopoulos; N K Kanellopoulos; A Ioffe Journal: J Invest Dermatol Date: 1998-06 Impact factor: 8.551
Authors: M A Kiselev; N Y Ryabova; A M Balagurov; S Dante; T Hauss; J Zbytovska; S Wartewig; R H H Neubert Journal: Eur Biophys J Date: 2005-07-20 Impact factor: 1.733
Authors: Steffi Hansen; Arne Naegel; Michael Heisig; Gabriel Wittum; Dirk Neumann; Karl-Heinz Kostka; Peter Meiers; Claus-Michael Lehr; Ulrich F Schaefer Journal: Pharm Res Date: 2009-02-25 Impact factor: 4.200