AIM: ABO-hemolytic disease occurs when ABO-incompatibility is present between the mother and the fetus. The objective of the present study was to analyze ABO-hemolytic disease in our center, to compare two serologic tests to diagnose it and to evaluate hematocrit values in cord blood samples. METHODS: ABO and Rh(D) group was found in 881 blood samples. Umbilical cord blood samples were obtained and direct antiglobulin test (DAT), eluate and microhematocrit were performed. RESULTS: ABO-incompatibility was found in 134 (15.3%) cases. DAT was positive in 31.6% of patients studied with ABO-incompatibility and the eluate identified the antibody in 68.4% of studied patients (p = 0.001). Hematocrit values were lower in the group with ABO-incompatibility than in the ABO-compatible group (44 +/- 7.8% vs. 46.5 +/- 6.5%; p = 0.029). CONCLUSIONS: Although severe ABO hemolytic disease is rare, ABO incompatibility is detected with current serologic tests and it causes a certain degree of hemolysis in newborn infants.
AIM: ABO-hemolytic disease occurs when ABO-incompatibility is present between the mother and the fetus. The objective of the present study was to analyze ABO-hemolytic disease in our center, to compare two serologic tests to diagnose it and to evaluate hematocrit values in cord blood samples. METHODS:ABO and Rh(D) group was found in 881 blood samples. Umbilical cord blood samples were obtained and direct antiglobulin test (DAT), eluate and microhematocrit were performed. RESULTS:ABO-incompatibility was found in 134 (15.3%) cases. DAT was positive in 31.6% of patients studied with ABO-incompatibility and the eluate identified the antibody in 68.4% of studied patients (p = 0.001). Hematocrit values were lower in the group with ABO-incompatibility than in the ABO-compatible group (44 +/- 7.8% vs. 46.5 +/- 6.5%; p = 0.029). CONCLUSIONS: Although severe ABO hemolytic disease is rare, ABO incompatibility is detected with current serologic tests and it causes a certain degree of hemolysis in newborn infants.