| Literature DB >> 1107028 |
C Bruton, R Jakes, T Atkinson.
Abstract
A procedure is presented for the purification of 3 g of homogeneous methionyl-tRNA synthetase and 1 g of homogeneous tyrosyl-tRNA synthetase from 50 kg batches of Escherichia coli. The procedure permits the isolation of many enzymes simultaneously and the elution positions of seven other aminoacyl-tRNA synthetases, catalase, rhodanese, phosphofructokinase, elongation factor Tu and cytochrome b-562 are indicated. The problems of extraction work on this scale are discussed.Entities:
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Year: 1975 PMID： 1107028 DOI： 10.1111/j.1432-1033.1975.tb02459.x
Source DB: PubMed Journal: Eur J Biochem ISSN： 0014-2956