BACKGROUND: The Wilms' tumor 1 (WT1) gene encodes a transcription factor critical in urogenital development. Using a new model of prostate cancer progression that permits comparison of the cellular and molecular properties of increasingly aggressive sublines of simian virus 40 large T-antigen-immortalized human prostate epithelial cells within the same lineage, the role of WT1 in tumorigenesis was investigated. METHODS AND RESULTS: Using RT-PCR and northern blotting, we identified a novel truncated WT1 transcript in these prostate cancer cell lines. This 2.1-kb transcript consisted of the coding region of the zinc-finger domain of WT1, together with a portion of intron 5 at the 5' end of the transcript. Furthermore, two peptides were detected by western blotting using antibodies to epitopes of the COOH terminus of WT1. Using RT-PCR, the 2.1-kb transcript was also detected in leukemia cell line K562, breast cancer cell line MCF7, and blood samples from patients with acute leukemia. CONCLUSION: These novel findings in both cell lines and patient-derived specimens suggest this new WT1 gene alteration has a potential role in the development of new diagnostic assays for some human malignancies.
BACKGROUND: The Wilms' tumor 1 (WT1) gene encodes a transcription factor critical in urogenital development. Using a new model of prostate cancer progression that permits comparison of the cellular and molecular properties of increasingly aggressive sublines of simian virus 40 large T-antigen-immortalized human prostate epithelial cells within the same lineage, the role of WT1 in tumorigenesis was investigated. METHODS AND RESULTS: Using RT-PCR and northern blotting, we identified a novel truncated WT1 transcript in these prostate cancer cell lines. This 2.1-kb transcript consisted of the coding region of the zinc-finger domain of WT1, together with a portion of intron 5 at the 5' end of the transcript. Furthermore, two peptides were detected by western blotting using antibodies to epitopes of the COOH terminus of WT1. Using RT-PCR, the 2.1-kb transcript was also detected in leukemia cell line K562, breast cancer cell line MCF7, and blood samples from patients with acute leukemia. CONCLUSION: These novel findings in both cell lines and patient-derived specimens suggest this new WT1 gene alteration has a potential role in the development of new diagnostic assays for some humanmalignancies.
Authors: G Dong; R Rajah; T Vu; A R Hoffman; R G Rosenfeld; C T Roberts; D M Peehl; P Cohen Journal: J Clin Endocrinol Metab Date: 1997-07 Impact factor: 5.958
Authors: S Barbaux; P Niaudet; M C Gubler; J P Grünfeld; F Jaubert; F Kuttenn; C N Fékété; N Souleyreau-Therville; E Thibaud; M Fellous; K McElreavey Journal: Nat Genet Date: 1997-12 Impact factor: 38.330
Authors: J Pelletier; W Bruening; C E Kashtan; S M Mauer; J C Manivel; J E Striegel; D C Houghton; C Junien; R Habib; L Fouser Journal: Cell Date: 1991-10-18 Impact factor: 41.582
Authors: C Englert; X Hou; S Maheswaran; P Bennett; C Ngwu; G G Re; A J Garvin; M R Rosner; D A Haber Journal: EMBO J Date: 1995-10-02 Impact factor: 11.598
Authors: Mara Artibani; Andrew H Sims; Joan Slight; Stuart Aitken; Anna Thornburn; Morwenna Muir; Valerie G Brunton; Jorge Del-Pozo; Linda R Morrison; Elad Katz; Nicholas D Hastie; Peter Hohenstein Journal: Sci Rep Date: 2017-03-27 Impact factor: 4.379