OBJECTIVE: To investigate variations in speed, duration and acceleration rate of the Cytospin 3 cytocentrifuge (Shandon Scientific Ltd., Astmoor, England) on the differential cell count of bronchoalveolar lavage (BAL) fluid samples. STUDY DESIGN: BAL fluid samples (n = 51) were cytocentrifuged at various combinations of speed (500, 1,200 and 2,000 rpm), acceleration rate (low, medium and high) and duration (5, 10, 15 and 20 minutes). The preparations were May-Grünwald-Giemsa stained and differentiated on 500 cells. Data were analyzed by mixed model repeated measurements ANOVA. RESULTS: The mean lymphocyte count was significantly higher at 1,200 rpm than at 500 rpm, whereas the macrophage count decreased. Between 1,200 and 2,000 rpm, the number of both cell types stabilized. Significantly higher numbers of lymphocytes were recorded at 10 and 15 minutes of cytocentrifugation than at 5 minutes. The acceleration rate did not influence the differential cell count. Seventeen BAL fluid samples were selected to test the diagnostic impact of cell damage using a validated computer program. In 1 of 17 samples the predicted diagnosis did not correspond between two different speeds (500 and 2,000 rpm). CONCLUSION: Variations in cytocentrifugation speed and duration affected the mean lymphocyte and macrophage counts of BAL fluid samples.
OBJECTIVE: To investigate variations in speed, duration and acceleration rate of the Cytospin 3 cytocentrifuge (Shandon Scientific Ltd., Astmoor, England) on the differential cell count of bronchoalveolar lavage (BAL) fluid samples. STUDY DESIGN: BAL fluid samples (n = 51) were cytocentrifuged at various combinations of speed (500, 1,200 and 2,000 rpm), acceleration rate (low, medium and high) and duration (5, 10, 15 and 20 minutes). The preparations were May-Grünwald-Giemsa stained and differentiated on 500 cells. Data were analyzed by mixed model repeated measurements ANOVA. RESULTS: The mean lymphocyte count was significantly higher at 1,200 rpm than at 500 rpm, whereas the macrophage count decreased. Between 1,200 and 2,000 rpm, the number of both cell types stabilized. Significantly higher numbers of lymphocytes were recorded at 10 and 15 minutes of cytocentrifugation than at 5 minutes. The acceleration rate did not influence the differential cell count. Seventeen BAL fluid samples were selected to test the diagnostic impact of cell damage using a validated computer program. In 1 of 17 samples the predicted diagnosis did not correspond between two different speeds (500 and 2,000 rpm). CONCLUSION: Variations in cytocentrifugation speed and duration affected the mean lymphocyte and macrophage counts of BAL fluid samples.
Authors: Johannes B J Scholte; Helke A van Dessel; Catharina F M Linssen; Dennis C J J Bergmans; Paul H M Savelkoul; Paul M H J Roekaerts; Walther N K A van Mook Journal: J Clin Microbiol Date: 2014-07-30 Impact factor: 5.948
Authors: Catharina F M Linssen; Jan A Jacobs; Foekje F Stelma; Walther N K A van Mook; Peter Terporten; Cornelis Vink; Marjolein Drent; Cathrien A Bruggeman; Annick Smismans Journal: Intensive Care Med Date: 2008-08-05 Impact factor: 17.440
Authors: Johannes B J Scholte; Johan I M van der Velde; Catharina F M Linssen; Helke A van Dessel; Dennis C J J Bergmans; Paul H M Savelkoul; Paul M H J Roekaerts; Walther N K A van Mook Journal: BMC Pulm Med Date: 2015-08-12 Impact factor: 3.317
Authors: Esther van Mastrigt; Salomé Zweekhorst; Bas Bol; Jeroen Tibboel; Joost van Rosmalen; Janneke N Samsom; André A Kroon; Johan C de Jongste; Irwin K M Reiss; Martin Post; Mariëlle W Pijnenburg Journal: PLoS One Date: 2018-01-18 Impact factor: 3.240
Authors: M J Vanspauwen; R M Schnabel; C A Bruggeman; M Drent; W N K A van Mook; D C J J Bergmans; C F M Linssen Journal: J Med Virol Date: 2013-07-16 Impact factor: 2.327