Literature DB >> 11064205

Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis.

S Narayanan1, S Selvakumar, R Aarati, S K Vasan, P R Narayanan.   

Abstract

Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter.

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Year:  2000        PMID： 11064205     DOI： 10.1111/j.1574-6968.2000.tb09392.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  4 in total

1.  Improved green fluorescent protein reporter gene-based microplate screening for antituberculosis compounds by utilizing an acetamidase promoter.

Authors:  Chartchai Changsen; Scott G Franzblau; Prasit Palittapongarnpim
Journal:  Antimicrob Agents Chemother       Date:  2003-12       Impact factor: 5.191

2.  Molecular characterization of AmiC, a positive regulator in acetamidase operon of Mycobacterium smegmatis.

Authors:  Arunkumar Venkatesan; Kannan Palaniyandi; Sujatha Narayanan
Journal:  Cell Stress Chaperones       Date:  2017-12-22       Impact factor: 3.667

3.  Fitness costs of rifampicin resistance in Mycobacterium tuberculosis are amplified under conditions of nutrient starvation and compensated by mutation in the β' subunit of RNA polymerase.

Authors:  Taeksun Song; Yumi Park; Isdore Chola Shamputa; Sunghwa Seo; Sun Young Lee; Han-Seung Jeon; Hongjo Choi; Myungsun Lee; Richard J Glynne; S Whitney Barnes; John R Walker; Serge Batalov; Karina Yusim; Shihai Feng; Chang-Shung Tung; James Theiler; Laura E Via; Helena I M Boshoff; Katsuhiko S Murakami; Bette Korber; Clifton E Barry; Sang-Nae Cho
Journal:  Mol Microbiol       Date:  2014-02-26       Impact factor: 3.501

4.  Tetracycline-inducible gene regulation in mycobacteria.

Authors:  Marian C J Blokpoel; Helen N Murphy; Ronan O'Toole; Siouxsie Wiles; Ellen S C Runn; Graham R Stewart; Douglas B Young; Brian D Robertson
Journal:  Nucleic Acids Res       Date:  2005-02-01       Impact factor: 16.971
  4 in total

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