Literature DB >> 11060103

Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens.

F Kong1, S Gordon, G L Gilbert.   

Abstract

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.

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Year:  2000        PMID： 11060103      PMCID： PMC87576     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

1.  Rapid cycle DNA amplification: time and temperature optimization.

Authors:  C T Wittwer; D J Garling
Journal:  Biotechniques       Date:  1991-01       Impact factor: 1.993

2.  Distinctions in DNA and protein profiles among clinical isolates of Mycoplasma pneumoniae.

Authors:  C J Su; S F Dallo; H Alderman; J B Baseman
Journal:  J Gen Microbiol       Date:  1991-12

3.  Direct sample polymerase chain reaction for the detection of Mycoplasma pneumoniae: a simple system for clinical application.

Authors:  C G Fink; S J Read; M Sillis
Journal:  Br J Biomed Sci       Date:  1995-03       Impact factor: 3.829

4.  Molecular distinctions among clinical isolates of Mycoplasma pneumoniae.

Authors:  C J Su; S F Dallo; J B Baseman
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

5.  Effect of heat denaturation of target DNA on the PCR amplification.

Authors:  C E Gustafson; R A Alm; T J Trust
Journal:  Gene       Date:  1993-01-30       Impact factor: 3.688

6.  Rapid cycle sequencing in an air thermal cycler.

Authors:  H Swerdlow; K Dew-Jager; R F Gesteland
Journal:  Biotechniques       Date:  1993-09       Impact factor: 1.993

7.  Detection of Mycoplasma pneumoniae and Mycoplasma genitalium in clinical samples by polymerase chain reaction.

Authors:  B de Barbeyrac; C Bernet-Poggi; F Fébrer; H Renaudin; M Dupon; C Bébéar
Journal:  Clin Infect Dis       Date:  1993-08       Impact factor: 9.079

8.  Possible origin of sequence divergence in the P1 cytadhesin gene of Mycoplasma pneumoniae.

Authors:  C J Su; S F Dallo; A Chavoya; J B Baseman
Journal:  Infect Immun       Date:  1993-03       Impact factor: 3.441

9.  Comparison of PCR, culture, and serological tests for diagnosis of Mycoplasma pneumoniae respiratory tract infection in children.

Authors:  J W Dorigo-Zetsma; S A Zaat; P M Wertheim-van Dillen; L Spanjaard; J Rijntjes; G van Waveren; J S Jensen; A F Angulo; J Dankert
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948

10.  Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.

Authors:  J H Tjhie; F J van Kuppeveld; R Roosendaal; W J Melchers; R Gordijn; D M MacLaren; J M Walboomers; C J Meijer; A J van den Brule
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948
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  17 in total

1.  Species-specific PCR for identification of common contaminant mollicutes in cell culture.

Authors:  F Kong; G James; S Gordon; A Zelynski; G L Gilbert
Journal:  Appl Environ Microbiol       Date:  2001-07       Impact factor: 4.792

Review 2.  Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections.

Authors:  K Loens; D Ursi; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

3.  Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization.

Authors:  Hui Wang; Fanrong Kong; Peter Jelfs; Gregory James; Gwendolyn L Gilbert
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

4.  Multiclonal origin of macrolide-resistant Mycoplasma pneumoniae isolates as determined by multilocus variable-number tandem-repeat analysis.

Authors:  Yang Liu; Xinye Ye; Hong Zhang; Xiaogang Xu; Minggui Wang
Journal:  J Clin Microbiol       Date:  2012-05-30       Impact factor: 5.948

5.  Culture-independent molecular subtyping of Mycoplasma pneumoniae in clinical samples.

Authors:  Roger Dumke; Paul Christian Lück; Christoph Noppen; Christoph Schaefer; Heike von Baum; Reinhard Marre; Enno Jacobs
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

6.  Sequence analysis of the p1 adhesin gene of Mycoplasma pneumoniae in clinical isolates collected in Beijing in 2008 to 2009.

Authors:  Fei Zhao; Bin Cao; Jing Li; Sufan Song; Xiaoxia Tao; Yudong Yin; Lihua He; Jianzhong Zhang
Journal:  J Clin Microbiol       Date:  2011-06-22       Impact factor: 5.948

7.  Clinical severity of Mycoplasma pneumoniae (MP) infection is associated with bacterial load in oropharyngeal secretions but not with MP genotype.

Authors:  Anna C Nilsson; Per Björkman; Christina Welinder-Olsson; Anders Widell; Kenneth Persson
Journal:  BMC Infect Dis       Date:  2010-02-25       Impact factor: 3.090

8.  Molecular profiles of group B streptococcal surface protein antigen genes: relationship to molecular serotypes.

Authors:  Fanrong Kong; Sonia Gowan; Diana Martin; Gregory James; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

Review 9.  Mycoplasma pneumoniae and its role as a human pathogen.

Authors:  Ken B Waites; Deborah F Talkington
Journal:  Clin Microbiol Rev       Date:  2004-10       Impact factor: 26.132

10.  Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness.

Authors:  Elektra Szewczuk; Kiran Thapa; Terry Anninos; Kenneth McPhie; Geoff Higgins; Dominic E Dwyer; Keith K Stanley; Jonathan R Iredell
Journal:  BMC Infect Dis       Date:  2010-05-11       Impact factor: 3.090
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