PURPOSE: A preferential loss of ERG b-wave was detected in a substrain of C57BL/10 mice. Electroretinographic and histologic techniques were used to investigate this hitherto unknown retinopathy. METHODS: ERGs were obtained from normal and affected C57BL/10. C57BL/6 mice served also as controls. a-Wave and c-wave analyses were performed. Microscopic investigations were done at two different ages. RESULTS: In the scotopic ERG, a severe reduction of the b-wave amplitude could be observed, whereas the a-wave was only moderately attenuated ("negative ERG"). With age, the a-wave amplitude further decreased, but the rate of reduction was comparable to normals. Oscillatory potentials were severely altered, and the photopic ERG was absent. The ERG c-wave was comparable to normal. ERG a-wave analysis also revealed a reduced maximum amplitude, but no significant difference of receptor sensitivity. Light microscopy revealed a thinning of all retinal layers but mostly of the outer plexiform layer. The number of photoreceptor nuclei was reduced by one third. Electron microscopy revealed a profound loss of ribbon-shaped synapses between rod and rod-bipolar cells and severely abnormal ribbons in the case of cones. CONCLUSIONS: The so-called negative ERG was associated with alterations in the synaptic layer between rods and rod bipolars. The absent cone ERG may be due to the altered cone-on-bipolar synapses. The overall thinning of the retina as well as the moderately reduced scotopic a-wave amplitude remain unexplained.
PURPOSE: A preferential loss of ERG b-wave was detected in a substrain of C57BL/10 mice. Electroretinographic and histologic techniques were used to investigate this hitherto unknown retinopathy. METHODS: ERGs were obtained from normal and affected C57BL/10. C57BL/6 mice served also as controls. a-Wave and c-wave analyses were performed. Microscopic investigations were done at two different ages. RESULTS: In the scotopic ERG, a severe reduction of the b-wave amplitude could be observed, whereas the a-wave was only moderately attenuated ("negative ERG"). With age, the a-wave amplitude further decreased, but the rate of reduction was comparable to normals. Oscillatory potentials were severely altered, and the photopic ERG was absent. The ERG c-wave was comparable to normal. ERG a-wave analysis also revealed a reduced maximum amplitude, but no significant difference of receptor sensitivity. Light microscopy revealed a thinning of all retinal layers but mostly of the outer plexiform layer. The number of photoreceptor nuclei was reduced by one third. Electron microscopy revealed a profound loss of ribbon-shaped synapses between rod and rod-bipolar cells and severely abnormal ribbons in the case of cones. CONCLUSIONS: The so-called negative ERG was associated with alterations in the synaptic layer between rods and rod bipolars. The absent cone ERG may be due to the altered cone-on-bipolar synapses. The overall thinning of the retina as well as the moderately reduced scotopic a-wave amplitude remain unexplained.
Authors: Luis Pérez De Sevilla Müller; Janelle Liu; Alexander Solomon; Allen Rodriguez; Nicholas C Brecha Journal: J Comp Neurol Date: 2013-08-01 Impact factor: 3.215
Authors: Neal S Peachey; Jillian N Pearring; Pasano Bojang; Matthew E Hirschtritt; Gwen Sturgill-Short; Thomas A Ray; Takahisa Furukawa; Chieko Koike; Andrew F X Goldberg; Yin Shen; Maureen A McCall; Scott Nawy; Patsy M Nishina; Ronald G Gregg Journal: J Neurophysiol Date: 2012-08-15 Impact factor: 2.714
Authors: Vasily Kerov; Joseph G Laird; Mei-Ling Joiner; Sharmon Knecht; Daniel Soh; Jussara Hagen; Sarah H Gardner; Wade Gutierrez; Takeshi Yoshimatsu; Sajag Bhattarai; Teresa Puthussery; Nikolai O Artemyev; Arlene V Drack; Rachel O Wong; Sheila A Baker; Amy Lee Journal: J Neurosci Date: 2018-06-06 Impact factor: 6.167
Authors: John R Hetling; Monica S Baig-Silva; Christopher M Comer; Machelle T Pardue; Dalia Y Samaan; Nasser M Qtaishat; David R Pepperberg; Thomas J Park Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol Date: 2005-01-13 Impact factor: 1.836