| Literature DB >> 11051553 |
M Okuda1, H F Horn, P Tarapore, Y Tokuyama, A G Smulian, P K Chan, E S Knudsen, I A Hofmann, J D Snyder, K E Bove, K Fukasawa.
Abstract
In animal cells, duplication of centrosomes and DNA is coordinated. Since CDK2/cyclin E triggers initiation of both events, activation of CDK2/cyclin E is thought to link these two events. We identified nucleophosmin (NPM/B23) as a substrate of CDK2/cyclin E in centrosome duplication. NPM/B23 associates specifically with unduplicated centrosomes, and NPM/B23 dissociates from centrosomes by CDK2/cyclin E-mediated phosphorylation. An anti-NPM/B23 antibody, which blocks this phosphorylation, suppresses the initiation of centrosome duplication in vivo. Moreover, expression of a nonphosphorylatable mutant NPM/ B23 in cells effectively blocks centrosome duplication. Thus, NPM/B23 is a target of CDK2/cyclin E in the initiation of centrosome duplication.Entities:
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Year: 2000 PMID： 11051553 DOI： 10.1016/s0092-8674(00)00093-3
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582