| Literature DB >> 1104600 |
K Kato, Y Hamaguchi, H Fukui, E Ishikawa.
Abstract
Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase conjugate could be used for immunoassay of insulin.Entities:
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Year: 1975 PMID： 1104600
Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387