Reversed-phase separation of achiral isomers by varying temperature and either gradient time or solvent strength.

The separation of several isomer pairs of widely varied structure was studied as a function of changes in temperature, gradient time, mobile phase pH, column type ("monomeric" vs. "polymeric" C18-silica) and organic solvent (methanol vs. acetonitrile). General conclusions are drawn which may prove useful in future attempts at the separation of these and other achiral isomers.