Literature DB >> 11043761

Rapid and simultaneous high-performance liquid chromatography assay of polyamines and monoacetylpolyamines in biological specimens.

G Taibi1, M R Schiavo, M C Gueli, P C Rindina, R Muratore, C M Nicotra.   

Abstract

A rapid, resolutive and reproducible reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for polyamines and acetylpolyamines by adopting pre-column derivatization with benzoyl chloride. In a single run lasting less than 15 min ten polyamines were separated as well as traces of benzoic acid, methylbenzoate and benzoic anhydride. These contaminants, produced during the derivatization reaction, were almost all eliminated by washing steps envisaged in the same procedure. This simple and sensitive method can be applied to routine determination of polyamines in biological samples. A fine application of this procedure to the determination of endogenous content of polyamines in chick embryo retina was reported.

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Year:  2000        PMID： 11043761     DOI： 10.1016/s0378-4347(00)00314-5

Source DB:  PubMed          Journal:  J Chromatogr B Biomed Sci Appl        ISSN： 1387-2273


  6 in total

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  6 in total

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