Literature DB >> 11043388

Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR.

K Specht1, T Richter, U Müller, A Walch, M W Höfler.   

Abstract

Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples.

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Year:  2000        PMID： 11043388

Source DB:  PubMed          Journal:  J Mol Med (Berl)        ISSN： 0946-2716            Impact factor:   4.599


  8 in total

1.  Optimizing gene expression analysis in archival brain tissue.

Authors:  Vivianna M D Van Deerlin; Lisa H Gill; Peter T Nelson
Journal:  Neurochem Res       Date:  2002-10       Impact factor: 3.996

2.  Towards quantitative mRNA analysis in paraffin-embedded tissues using real-time reverse transcriptase-polymerase chain reaction: a methodological study on lymph nodes from melanoma patients.

Authors:  Helene Nortvig Abrahamsen; Torben Steiniche; Ebba Nexo; Stephen J Hamilton-Dutoit; Boe Sandahl Sorensen
Journal:  J Mol Diagn       Date:  2003-02       Impact factor: 5.568

3.  Evidence for qualified quantitative mRNA analysis in formalin-fixed and paraffin-embedded colorectal carcinoma cells and tissue.

Authors:  Xiaobo Lu; Tahar van der Straaten; Marco Tiller; Xiaoxia Li
Journal:  J Clin Lab Anal       Date:  2011       Impact factor: 2.352

4.  High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue.

Authors:  Alice Ly; Achim Buck; Benjamin Balluff; Na Sun; Karin Gorzolka; Annette Feuchtinger; Klaus-Peter Janssen; Peter J K Kuppen; Cornelis J H van de Velde; Gregor Weirich; Franziska Erlmeier; Rupert Langer; Michaela Aubele; Horst Zitzelsberger; Liam McDonnell; Michaela Aichler; Axel Walch
Journal:  Nat Protoc       Date:  2016-07-14       Impact factor: 13.491

5.  The predictive value of genes of the TGF-beta1 pathway in multimodally treated squamous cell carcinoma of the esophagus.

Authors:  Franziska Pühringer-Oppermann; Mario Sarbia; Nicola Ott; Björn L D M Brücher
Journal:  Int J Colorectal Dis       Date:  2009-12-15       Impact factor: 2.571

6.  Using Q-RT-PCR to measure cyclin D1, TS, TP, DPD, and Her-2/neu as predictors for response, survival, and recurrence in patients with esophageal squamous cell carcinoma following radiochemotherapy.

Authors:  Björn L D M Brücher; Gisela Keller; Martin Werner; Ulrike Müller; Silke Lassmann; Antonello Domenico Cabras; Falko Fend; Raymonde Busch; Hubert Stein; Hans-Dieter Allescher; Michael Molls; J Rüdiger Siewert; Heinz Höfler; Katja Specht
Journal:  Int J Colorectal Dis       Date:  2008-08-13       Impact factor: 2.571

7.  A high-throughput method to detect RNA profiling by integration of RT-MLPA with next generation sequencing technology.

Authors:  Jing Wang; Xue Yang; Haofeng Chen; Xuewei Wang; Xiangyu Wang; Yi Fang; Zhenyu Jia; Jidong Gao
Journal:  Oncotarget       Date:  2017-07-11

8.  Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples.

Authors:  Clemens L Bockmeyer; Juliane Wittig; Karen Säuberlich; Philipp Selhausen; Marc Eßer; Philip Zeuschner; Friedrich Modde; Kerstin Amann; Christoph Daniel
Journal:  BMC Mol Biol       Date:  2018-03-13       Impact factor: 2.946
  8 in total

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