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Literature DB >> 11036056

Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli.

S J Kopytek¹, J C Dyer, G S Knapp, J C Hu.

Abstract

Many laboratory strains of Escherichia coli are resistant to methotrexate (MTX), a folate analogue that binds dihydrofolate reductase (DHFR). Mutations that inactivate either tolC or acrA confer MTX sensitivity. Further, overexpression of a fusion protein with DHFR activity reverses this sensitivity by titrating out intracellular MTX. These results suggest that MTX accumulates in cells where mutations in acrA or tolC have inactivated the TolC-dependent AcrAB multidrug resistance efflux pump.

Entities: Chemical Gene Species

Mesh：

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Year: 2000 PMID： 11036056 PMCID： PMC101636 DOI： 10.1128/AAC.44.11.3210-3212.2000

Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN： 0066-4804 Impact factor: 5.191

26 in total

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