Literature DB >> 11017709

Separation and quantitation of phospholipids and lysophospholipids by high-performance liquid chromatography.

E J Lesnefsky1, M S Stoll, P E Minkler, C L Hoppel.   

Abstract

We describe a comprehensive approach to the separation, quantitation, and characterization of phospholipids and lysophospholipids present in complex biological samples. The central feature is a normal-phase HPLC separation of individual phospholipid and lysophospholipid classes. In this single chromatographic step, phospholipids and lysophospholipids are separated and recovered for quantitation by organic phosphate assay and characterization by acyl-group composition. Recovery of phospholipids and lysophospholipids from HPLC averages 80-90%. Isolated phospholipid and lysophospholipid fractions are available for separation of individual molecular species by second-dimension reverse-phase HPLC and characterization of individual molecular species by mass spectrometry. Copyright 2000 Academic Press.

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Year:  2000        PMID： 11017709     DOI： 10.1006/abio.2000.4783

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  20 in total

1.  Characterization of cardiolipin as the sodiated ions by positive-ion electrospray ionization with multiple stage quadrupole ion-trap mass spectrometry.

Authors:  Fong-Fu Hsu; John Turk
Journal:  J Am Soc Mass Spectrom       Date:  2006-06-05       Impact factor: 3.109

2.  Structural characterization of cardiolipin by tandem quadrupole and multiple-stage quadrupole ion-trap mass spectrometry with electrospray ionization.

Authors:  Fong-Fu Hsu; John Turk; Elizabeth R Rhoades; David G Russell; Yixin Shi; Eduardo A Groisman
Journal:  J Am Soc Mass Spectrom       Date:  2005-04       Impact factor: 3.109

3.  Characterization of cardiolipin from Escherichia coli by electrospray ionization with multiple stage quadrupole ion-trap mass spectrometric analysis of [M - 2H + Na]- ions.

Authors:  Fong-Fu Hsu; John Turk
Journal:  J Am Soc Mass Spectrom       Date:  2006-01-25       Impact factor: 3.109

4.  An improved method for separating cardiolipin by HPLC.

Authors:  Gwendolyn Barceló-Coblijn; Eric J Murphy
Journal:  Lipids       Date:  2008-07-18       Impact factor: 1.880

5.  Cardiolipin as an oxidative target in cardiac mitochondria in the aged rat.

Authors:  Edward J Lesnefsky; Charles L Hoppel
Journal:  Biochim Biophys Acta       Date:  2008-06-02

6.  Permeabilization of the mitochondrial outer membrane by Bax/truncated Bid (tBid) proteins as sensitized by cardiolipin hydroperoxide translocation: mechanistic implications for the intrinsic pathway of oxidative apoptosis.

Authors:  Witold Korytowski; Liana V Basova; Anna Pilat; Robert M Kernstock; Albert W Girotti
Journal:  J Biol Chem       Date:  2011-06-03       Impact factor: 5.157

7.  Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS.

Authors:  Honggang Nie; Ranran Liu; Youyou Yang; Yu Bai; Yafeng Guan; Daqing Qian; Tao Wang; Huwei Liu
Journal:  J Lipid Res       Date:  2010-06-06       Impact factor: 5.922

8.  Electron flow into cytochrome c coupled with reactive oxygen species from the electron transport chain converts cytochrome c to a cardiolipin peroxidase: role during ischemia-reperfusion.

Authors:  Hema S Aluri; David C Simpson; Jeremy C Allegood; Ying Hu; Karol Szczepanek; Scott Gronert; Qun Chen; Edward J Lesnefsky
Journal:  Biochim Biophys Acta       Date:  2014-08-01

Review 9.  Lipidomic analysis of cerebrospinal fluid by mass spectrometry-based methods.

Authors:  Benoit Colsch; Alexandre Seyer; Samia Boudah; Christophe Junot
Journal:  J Inherit Metab Dis       Date:  2014-12-09       Impact factor: 4.982

10.  Separation and characterization of cardiolipin molecular species by reverse-phase ion pair high-performance liquid chromatography-mass spectrometry.

Authors:  Paul E Minkler; Charles L Hoppel
Journal:  J Lipid Res       Date:  2009-10-30       Impact factor: 5.922
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