[Utilization of LIN (lysine-indole-motility) medium for the preliminary identification of enteropathogenic bacteria].

A multiple-test medium for the routine laboratory identification of enteropathogenic bacteria is described. The medium has the following formula: Bacto-peptone (Difco) 5 g; yeast extract (Difco) 3 g; casein tryptic digest peptone (Merek) 15 g; glucose 1 g; L-lysine-monohydrochloride (Merck, No. 5700) 5 g; NaCl 5 g; bromcresol purple 0.016 g; agar 2 g; distilled water 1000 ml; final pH 6.6. The medium is dispensed in amounts of 5 ml into tubes of 14 X 85 mm and autoclaved at 120 degrees C for 10 min. The tubes are tightly closed with rubber stoppers. - The medium is inoculated by stabbing to the bottom of the tube. Readings are made after over-night incubation at 37 degrees C. A scheme for the preliminary identification of enteropathogenic bacteria is given, based on LIM medium in conjunction with Kligler's iron agar, and the oxidase reaction.