Literature DB >> 11011086

Elimination of HIV-1 plasmid DNA from virus samples obtained from transfection by calcium-phosphate co-precipitation.

K B Koh1, M Fujita, A Adachi.   

Abstract

Molecular genetics is a powerful tool to analyze the replication cycle of human immunodeficiency virus type 1 (HIV-1). Culture fluids obtained from HIV-1 plasmid-transfected cells by calcium-phosphate co-precipitation were treated with ethyleneglycol bis (beta-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and DNase I to obtain HIV-1 stocks virtually free of input plasmid DNAs. Even after amplification by polymerase chain reaction (PCR), no plasmid DNA was detected in cells following infection with EGTA/DNase I-treated virus samples. This method is particularly useful for the examination of the early replication phase of HIV-1 by PCR.

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Year:  2000        PMID： 11011086     DOI： 10.1016/s0166-0934(00)00224-x

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  7 in total

1.  Vpx is critical for reverse transcription of the human immunodeficiency virus type 2 genome in macrophages.

Authors:  Mikako Fujita; Masami Otsuka; Masami Miyoshi; Boonruang Khamsri; Masako Nomaguchi; Akio Adachi
Journal:  J Virol       Date:  2008-05-21       Impact factor: 5.103

2.  The human immunodeficiency virus type 2 Vpx protein usurps the CUL4A-DDB1 DCAF1 ubiquitin ligase to overcome a postentry block in macrophage infection.

Authors:  Anna Bergamaschi; Diana Ayinde; Annie David; Erwann Le Rouzic; Marina Morel; Gilles Collin; Diane Descamps; Florence Damond; Françoise Brun-Vezinet; Sebastien Nisole; Florence Margottin-Goguet; Gianfranco Pancino; Catherine Transy
Journal:  J Virol       Date:  2009-03-04       Impact factor: 5.103

3.  Adenovirus vectors block human immunodeficiency virus-1 replication in human alveolar macrophages by inhibition of the long terminal repeat.

Authors:  Robert J Kaner; Francisco Santiago; Franck Rahaghi; Elizabeth Michaels; John P Moore; Ronald G Crystal
Journal:  Am J Respir Cell Mol Biol       Date:  2009-10-05       Impact factor: 6.914

4.  Minimal region sufficient for genome dimerization in the human immunodeficiency virus type 1 virion and its potential roles in the early stages of viral replication.

Authors:  Jun-Ichi Sakuragi; Sayuri Sakuragi; Tatsuo Shioda
Journal:  J Virol       Date:  2007-05-16       Impact factor: 5.103

5.  The relationship between HIV-1 genome RNA dimerization, virion maturation and infectivity.

Authors:  Masahisa Ohishi; Takashi Nakano; Sayuri Sakuragi; Tatsuo Shioda; Kouichi Sano; Jun-ichi Sakuragi
Journal:  Nucleic Acids Res       Date:  2010-12-23       Impact factor: 16.971

6.  A proposal for a new HIV-1 DLS structural model.

Authors:  Jun-ichi Sakuragi; Hirotaka Ode; Sayuri Sakuragi; Tatsuo Shioda; Hironori Sato
Journal:  Nucleic Acids Res       Date:  2012-02-10       Impact factor: 16.971

7.  Absence of cGAS-mediated type I IFN responses in HIV-1-infected T cells.

Authors:  Carina Elsner; Aparna Ponnurangam; Julia Kazmierski; Thomas Zillinger; Jenny Jansen; Daniel Todt; Katinka Döhner; Shuting Xu; Aurélie Ducroux; Nils Kriedemann; Angelina Malassa; Pia-Katharina Larsen; Gunther Hartmann; Winfried Barchet; Eike Steinmann; Ulrich Kalinke; Beate Sodeik; Christine Goffinet
Journal:  Proc Natl Acad Sci U S A       Date:  2020-07-24       Impact factor: 11.205
  7 in total

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