Study of the fluorescence characteristics of norfloxacin in reversed micelles and application in analysis.

The fluorescence characteristics of norfloxacin (NFLX) were studied in reversed micelles which formed with sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-water-octane. The influences of the environmental factors, such as the water content, AOT concentration and pH, on the fluorescence of NFLX were investigated. A novel spectrofluorimetric method for the direct determination of NFLX is proposed based on the enhancement effect of reversed micelles on the native fluorescence of NFLX, which showed a much lower detection limit (0.0032 microgram mL-1) and a wider linear range (0.015-3.8 micrograms mL-1) compared with aqueous solution.